Banca de QUALIFICAÇÃO: MARIANA CABRAL MORENO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARIANA CABRAL MORENO
DATA : 27/05/2019
HORA: 08:00
LOCAL: Departamento de Odontologia
TÍTULO:

EFFECT OF THERMOGENIC SUBSTANCES ON BEHAVIOR AND ORTHODONTIC MOVEMENT IN RATS: AN IN VIVOEXPERIMENTAL STUDY

 

 

 

 

PALAVRAS-CHAVES:

Orthodontics, Tooth Movement Techniques, Rats.

PÁGINAS: 31
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Ortodontia
RESUMO:

INTRODUCTION: The thermogenic supplement presents high concentration of caffeine as the main substance associated with other compounds; accelerates the basal metabolism and assists in burning body fat/weight loss. However, the evidence is still non-existent regarding its effect on orthodontic movement (OM). PURPOSE: To elucidate the effect of thermogenic C4 Beta Pump and caffeine on the behavior, amount of tooth movement, root resorption and bone remodeling in rats submitted to OM. METHODS: In this experimental study in vivo, 36 healthy male Wistar rats (Ratus norvegicus albinus) between 10-12 weeks, 200-320g were used. They will be submitted to MO (closed spring of nickel-titanium activated between 1st molar and upper incisors of the left side) and daily administration of the substances by gavage for 21 days. They will be distributed in three groups of 12 animals: control group (CG) - submitted to OM and water; experimental group 1 (G1) - submitted to OM and thermogenic C4; experimental group 2 (G2) - submitted to OM and caffeine. The open field test will be used in the four-stroke behavioral evaluation: baseline (T0), after placement of the springs (T1), after the first gavage (T2), after 21 days (T3). The amount of OM will be verified through a digital caliper and micro computed tomography (µCT). For root resorption will be used tartrate resistant acid phosphatase staining (TRAP) for osteoclast counting. As for bone remodeling will be performed immunohistochemical analysis for monoclonal antibodies from rats to Osteoprotegerin (OPG), RANK, RANK-L, IL-1β, IL-6, TNF-α. Then the ANOVA statistical test a factor will be used at a significance level of 5%.

 

MEMBROS DA BANCA:
Presidente - 2665455 - HALLISSA SIMPLICIO GOMES PEREIRA
Interna - 2492713 - ERICKA JANINE DANTAS DA SILVEIRA
Externo ao Programa - 1378325 - ARTHUR CESAR DE MEDEIROS ALVES