EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF A FLOWABLE BULK FILL RESIN COMPOSITE AFTER THE INSERTION OF SCHINOPSIS BRASILIENSIS (BRAÚNA) LEAF EXTRACT
Composite resins. Phytotherapy. Antimicrobial action.
Introduction: The insertion of antimicrobial herbal extracts in restorative materials has been an alternative to generate antimicrobial capacity for them. Objective: To evaluate the antimicrobial action of Opus Bulk Fill Flow APS resin with different concentrations of Schinopsis brasiliensis (Braúna) leaf extract. Materials and methods: An in vitro experimental study will be carried out. Initially, the Braúna Leaf Extract (EFB) will be obtained from the drying of the leaves (40 ± 1 °C) and then the percolation of its active principle. Afterwards, the EFB will be added to the Opus Bulk Fill Flow APS® (FGM) resin at concentrations of 0 (control), 1 mg/ml, 3 mg/ml, 6 mg/ml, and 9 mg/ml. In order to evaluate the antimicrobial activity, 50 circular specimens (5 mm x 1 mm) (n = 10 per group) will be made using a silicone mold Express XT. After preparation, the specimens will be placed at the bottom of wells in 48-well plates and covered with culture medium containing the inoculum (BHI broth and saliva). For the positive control group, 0.12% Chlorhexidine Digluconate will be used. Then, the plates will be incubated at 37 ± 1 ˚C for 24 hours. After the period, 10μL aliquots of each well will be removed, seeded in a Petri dish containing BHI agar (in triplicate) and incubated for 24 h at 37 ºC to evaluate the microbial growth. The count of Colony Forming Units (CFU) will be performed to verify viability.