Banca de DEFESA: BRUNA LORENA MENESES MARQUES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BRUNA LORENA MENESES MARQUES
DATE: 15/12/2023
TIME: 09:00
LOCAL: Link de acesso para videoconferência: meet.google.com/rch-txvs-bwv
TITLE:
NANOPARTICLES CONTAINING QUINOA OIL (CHENOPODIUM QUINOA WILLD.): SYNTHESIS, PHYSICOCHEMICAL CHARACTERIZATION, IN VITRO TOXICITY EVALUATION, AND BIOACTIVE POTENTIAL

KEY WORDS:
Nanoencapsulation; Cell viability; Total Antioxidant Capacity; Antibacterial activity; α-amylase and amylglucosidase; HaCat; NIH-3T3.

PAGES: 98
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
Quinoa oil (Chenopodium quinoa Willd.) is rich in unsaturated fatty acids and vitamin
E. Nanoencapsulation of lipophilic components has emerged as a promising strategy
for solubilization in an aqueous matrix, preservation of bioactive compounds, improved
incorporation in processed products, increased bioavailability, and preservation of the
oil's bioactive properties. The aim of this study was to encapsulate quinoa oil,
characterize the formulations, and evaluate the bioactive potential of the oil and
encapsulated forms. Nanoemulsions were produced using an oil-in-water
emulsification technique, with swine gelatin (OG) and whey protein isolate (OPG) as
encapsulating agents and Tween 20 as a surfactant. Characterization of the
nanoparticles was performed using techniques such as Scanning Electron Microscopy
(SEM), Dynamic Light Scattering (DLS), Fourier Transform Infrared Spectroscopy
(FTIR), Dispersibility Assay, Thermogravimetry (TG), Differential Scanning
Calorimetry (DSC), evaluation of Zeta Potential at different pH values, encapsulation
efficiency (%), and dispersibility in water. The cytotoxicity of free and
nanoencapsulated quinoa oil was evaluated in vitro using Chinese hamster ovary
(CHO) and human hepatocarcinoma (HepG2) cells through the 3-(4,5-dimethylthiazol-
2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidant capacity was
determined by Total Antioxidant Capacity (TAC) and metal ion chelation tests.
Antibacterial activity was assessed by determining the Minimum Inhibitory
Concentration (MIC) against Gram-negative and Gram-positive bacteria. Inhibition of
glucose-metabolizing enzymes was investigated using α-amylase and
amylglucosidase inhibition tests. Additionally, tests of activity in epithelial cells were
carried out using the Neutral Red assay with HaCat and NIH-3T3 cells. SEM analysis
showed smooth and crack-free surfaces of the OG and OPG nanoparticles, while DLS
revealed an average particle size ranging from 160 to 264 nm. The encapsulation
efficiency was 74.137% for OPG and 83.085% for OG nanoformulations. Cytotoxicity
analysis revealed that both free and nanoencapsulated quinoa oil did not exhibit
cytotoxic effects on CHO and HepG2 cells, with a cell viability higher than 70%.
Regarding antioxidant activity, the nanoformulations showed greater activity than pure
oil. There was no inhibition of bacterial growth at the tested concentrations for all
samples. The nanoformulations OG and OPG demonstrated greater capacity to inhibit
α-amylase and amylglucosidase enzymes compared crude oil. In the viability assay
with epithelial cells, the nanoformulations showed cell viability above 70% at most tested concentrations in the Neutral Red assay. Quinoa oil and OPG showed
increased cell proliferation in the MTT assay (approximately 50%). These results
indicate that encapsulation of quinoa oil enhances its biological properties and shows
potential for future use in pharmaceutical, food, or cosmetic industries.

COMMITTEE MEMBERS:
Presidente - 2378605 - CRISTIANE FERNANDES DE ASSIS
Externa ao Programa - 3315429 - LUCIANA GUIMARAES ALVES FILGUEIRA - nullExterno à Instituição - SÉRGIO DANTAS DE OLIVEIRA JÚNIOR - INPA
Notícia cadastrada em: 29/11/2023 11:28
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