Banca de QUALIFICAÇÃO: GABRIELA ROCHA RAMOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : GABRIELA ROCHA RAMOS
DATE: 30/11/2023
TIME: 09:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/zzr-tyjo-iqg
TITLE:

Obtaining a Biotechnological Article for Application in Cosmetic Formulations

KEY WORDS:

Lipase. Aspergillus terreus. Immobilization. Characterization. Cosmetic.

PAGES: 109
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Lipases are important enzymes belonging to the class of hydrolases that are prominent in biotechnological experiments. The aim of this work was to produce the lipase enzyme from Aspergillus terreus using solid state fermentation and carry out its characterization and immobilization in silica gel for incorporation into cosmetics. Furthermore, this work also evaluated the enzymatic activity and physicochemical characterization of commercial lipase from Rhizopus oryzae. Lipase production was carried out using the fungus Aspergillus terreus and olive oil as an inducing agent. The enzymatic activity was carried out using the p-nitrophenyl-palmitate (pNpp) methodology and the immobilization of the lipase produced was carried out by physical adsorption using silica gel as a support. The enzymes were characterized using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Diffraction (XRD) and safety assessment through cytotoxicity tests using NIH-3T3 fibroblast cells. A central rotational composite design was made to find the best conditions for the production of the enzymatic cosmetic. The enzyme produced by A. terreus showed an activity of 375.9 U/g and the immobilized enzyme of 99.6 U/g. The SEM poor interaction between enzyme and silica during immobilization, which was confirmed by FTIR and XRD. The cytotoxicity test showed that only the lipase produced by A. terreus was safe for NIH-3T3 fibroblast cells. Tests to evaluate lipolytic performance at different temperatures showed that the optimal temperature for enzymatic activity is 37º C and pH optimal is 7.0. The central rotational composite design shows that the agitation time and enzyme concentration influenced the zeta potential response, but only the agitation time influenced the enzymatic activity response. According to the results, the enzyme produced by the fungus A. terreus is promising and safe for use in cosmetics.

COMMITTEE MEMBERS:
Externo ao Programa - 3304882 - CARLOS EDUARDO DE ARAÚJO PADILHA - nullPresidente - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Interno - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA