Banca de QUALIFICAÇÃO: BRUNA LORENA MENESES MARQUES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : BRUNA LORENA MENESES MARQUES
DATE: 31/08/2023
TIME: 14:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/eod-bsdm-kqt
TITLE:

NANOPARTICLES CONTAINING QUINOA OIL (Chenopodium quinoa WILLD.): SYNTHESIS, PHYSICOCHEMICAL CHARACTERIZATION, IN VITRO CITOTOXICITY EVALUATION, AND BIOACTIVE POTENTIAL


KEY WORDS:
Nanoencapsulation; Cell viability; Total Antioxidant Capacity; Antimicrobial activity; Antidiabetic activity.

PAGES: 117
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
Quinoa (Chenopodium quinoa Willd.) is a pseudocereal recognized for its superior
nutritional value compared to traditional cereals, high content of micronutrients, and
protein richness. Quinoa oil is rich in unsaturated fatty acids, along with significant
amounts of vitamin E. Nanoencapsulation of lipophilic components is a promising
strategy to promote solubilization in aqueous matrices, preserve bioactive compounds,
improve incorporation into processed products, increase bioavailability, as well as
preserve and enhance the bioactive properties of the oil. In order to apply quinoa oil
in pharmaceutical, food, and/or cosmetic matrices, it was nanoencapsulated, and its
toxicity and bioactive potential were evaluated. The nanoparticles were synthesized
using O/W emulsification and multilayer techniques, with porcine gelatin (OG) and
whey protein (OPG) as encapsulating agents, and Tween 20 as a surfactant. The
nanoparticles were characterized using Dynamic Light Scattering (DLS), Fourier
Transform Infrared Spectroscopy (FTIR), Thermogravimetry (TG), Differential
Scanning Calorimetry (DSC), as well as evaluation of the Zeta Potential at different
pH values and encapsulation efficiency (%). The cytotoxicity of free and
nanoencapsulated quinoa oil was assessed in vitro using Chinese hamster ovary
(CHO) cells through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
(MTT) assay. The antioxidant potential of quinoa oil and nanoformulations was
determined through total antioxidant capacity (TAC) and metal ion chelation tests. The
modulatory antimicrobial activity was evaluated by determining the minimum inhibitory
concentration (MIC) against Gram-negative and Gram-positive bacteria. Moreover,
the in vitro antidiabetic activity was investigated through α-amylase and amylase tests.
The data obtained from particle characterization revealed average particle sizes
ranging from 160 to 314 nm. FTIR analysis indicated chemical interactions between
the materials used, as evidenced by vibrational band shifts and the formation of new
bands, confirming the interaction between quinoa oil and porcine gelatin, thus
reinforcing the effectiveness of encapsulation. The encapsulation efficiency achieved
was 74.137% and 83.085% for OPG and OG nanoformulations, respectively. The
cytotoxicity analysis revealed that both free and nanoencapsulated quinoa oil did not
exhibit cytotoxic effects on CHO cells. Regarding antioxidant activity, the
nanoformulations showed higher activity compared to pure oil. The MIC for both Gram
positive and Gram-negative bacteria, for both crude quinoa oil and produced nanoformulations, was above 10 mg/mL. Regarding antidiabetic activity, the two
nanoformulations at a concentration of 10 mg/mL demonstrated superior inhibition
capacity of α-amylase and amylase enzymes compared to crude oil. These results
indicate that nanoencapsulation of quinoa oil holds potential for various biological
applications.

COMMITTEE MEMBERS:
Interno - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Presidente - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externo à Instituição - FRANCISCO HUMBERTO XAVIER JUNIOR - UFPB
Notícia cadastrada em: 15/08/2023 11:55
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