Banca de DEFESA: KYVIA REGINA FERNANDES VARELA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KYVIA REGINA FERNANDES VARELA
DATE: 29/11/2022
TIME: 09:00
LOCAL: Link de acesso para videoconferência: meet.google.com/iim-vdyf-dfn
TITLE:

DESENVOLVIMENTO DE NANOFORMULAÇÕES DE PROTÓTIPOS DE VACINAS DE DNA E AVALIAÇÃO DE PARÂMETROS FÍSICO-QUÍMICOS E BIOLÓGICOS


KEY WORDS:
Plasmids; DNA vacines; Polymeric nanoparticles; Polyethyleneimine; Gene Transfer; Nucleases.

PAGES: 98
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
DNA vacines, also called genetic vacines, are based on the use of plasmids that encode
pathogen gene sequences as an antigenic candidate for inducing immunity. These vaccines have
numerous advantages when compared to other vaccines; are easy to develop, low cost of
production, safety and induction of cellular and humoral immune response. However, its main
disadvantage is a reduced rate of cell transfection and consequently low endogenous production
of the antigen of interest. This disadvantage is mainly due to the degradation of plasmids by
nucleases present in tissues and inside cells. Thus, this study aims to develop a nanosystem
containing plasmids used in DNA vaccine models. Specifically plasmids pVAX1, pVAX1lacZ
and nTSApVAX1, incorporated into polymeric polylactic acid (PLA) nanoparticles, with and
without the presence of polyethyleneimine (PEI), which can protect and increase the levels of
gene transfer of the plasmids, thus, building efficient vaccine prototypes. Therefore, after
formulation, the physicochemical parameters of the systems were evaluated from the mean
particle diameter, polydispersity index (PDI) and zeta potential. Additionally, performance tests
were performed, such as: physical-chemical stability, electrophoretic mobility delay, protection
against enzymatic degradation on different sources and types of enzymes, and in vitro cell
viability. The nanoformulations produced had an average size around 250 nm, PDI <0.3. The
cationic nanoformulations (PLA + PEI + pDNA) showed good complexation between polymers
and pDNA, as there was no appearance of bands in the electrophoresis assays, even against the
degradation enzymes, also suggesting protection. The same profile was not observed with the
anionic nanoformulations (PLA + pDNA), in which bands referring to the pDNAs appeared,
indicating a not so efficient binding between the componentes. Moreover, all systems showed
good physicalchemical stability during a six weeks period, as well asan adequate cell viability
(>70%), not showing any relevant citotoxicity. Thus, nanoformulated pDNAs are shown to be
promising and safe carriers in the development of DNA vaccine prototypes.

COMMITTEE MEMBERS:
Presidente - 2275890 - MARCELO DE SOUSA DA SILVA
Externa ao Programa - 2085604 - SUSANA MARGARIDA GOMES MOREIRA - nullExterna à Instituição - WENDY MARINA TOSCANO QUEIROZ DE MEDEIROS - IFRN
Notícia cadastrada em: 11/11/2022 17:22
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