Banca de DEFESA: ANNAMAIRLLA DO NASCIMENTO OLIVEIRA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANNAMAIRLLA DO NASCIMENTO OLIVEIRA
DATE: 28/11/2022
TIME: 09:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/imy-aibx-pqz
TITLE:
AVALIAÇÃO DA RESPOSTA IMUNE HUMORAL EM ANIMAIS IMUNIZADOS COM ANTÍGENOS TOTAIS DE Leishmania amazonensis ASSOCIADOS A DIFERENTES ADJUVANTES VACINAIS
KEY WORDS:
Leishmania amazonensis. Adjuvants. VPs. Immunization.
PAGES: 101
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
Neglected tropical diseases (NTDs) are a major public health challenge. Leishmaniasis, a
classic example of these NTDs, affects millions of people around the world, especially those
in poverty and vulnerability. Its treatment consists of the use of toxic and expensive drugs.
Currently, the means of preventing leishmaniasis is through vector control. However, such
measures are inefficient, making it necessary to develop a more safe and effective alternative.
In this scenario, the vaccine presents themselves an alternative, not only for prophylaxis, but
also for treatment. Therefore, the present study aimed to characterize the immunostimulatory
capacity of total Leishmania amazonensis antigens, parallel to the evaluation of the Triatoma
virus’s structural proteins (VP1, VP2, and VP3) immunoadjuvant potential comparing their
activity to adjuvants estabilished in the literature, such as: Freud’s adjuvante and aluminum
hydroxide. Initially, promastigotes of L. amazonensis were cultivated; a total protein extract
was obtained and the electrophoretic profile was characterized. After these processes,
BALB/c mice (Mus musculus) were divided into five groups: PBS (negative control), L.
amazonensis extract, extract + Freund's adjuvant, extract + aluminum hydroxide, and finally,
extract + VPs. The groups were submitted to primary (100µg) and secondary (100µg)
immunization, with an interval of 30 days between them. Serum samples were obtained 15
and 30 days after the primary immunization, and 7, 15, 30, 40, 50 and 60 days after the
booster dose. After this process, the characterization of the humoral immune response was
carried out, in which the profile of specific antibodies total IgG a and its isotypes IgG1,
IgG2a, IgG2b and IgG3 anti-L. amazonensis was taken into account, as well as the profile of
total IgG anti-Structural Proteins (VP1, VP2 and VP3). To characterize the cellular immune
response, the Delayed Hypersensitivity Reaction (DTH) was initially performed, followed by
the immunoproliferation assay to quantify cytokines. The results obtained demonstrated the
immunostimulatory character of the adjuvants used in this research, as well as the
immunomodulatory potential of the studied VPs, since the analyzes demonstrated their
potential to preferentially induce IgG2b and IgG3 titers when associated with the L.
amazonensis extract. The profile of IgG3 anti-Leishmania amazonensis antibodies was more
pronounced in the group of animals immunized with VPs when compared to the other groups
in the study. The titers of total IgG anti-structural proteins showed the highest titers of this
immunoglobulin for VP1, which suggests its potentiated immunoadjuvant activity against
VP2 and VP3 when associated with the total extract of L. amazonensis. Through the analysis
of the DTH assay, the immunostimulatory potential of the vaccine formulations proposed by this study is suggested. Taken together, the results obtained show promise for the elucidation
of VPs as adjuvants in vaccine preparations. However, future studies will allow attesting to
the present results.
COMMITTEE MEMBERS:
Externo à Instituição - DIEGO MARCELO GUERIN AGUILAR - UPV/EHU
Externo à Instituição - GABRIELA SANTOS GOMES - NOVA
Externo à Instituição - LUIZ FELIPE DOMINGUES PASSERO - UNESP
Presidente - 2275890 - MARCELO DE SOUSA DA SILVA