Banca de DEFESA: TAMYRES BERNADETE DANTAS QUEIROGA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : TAMYRES BERNADETE DANTAS QUEIROGA
DATE: 19/10/2022
TIME: 09:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/awf-quzs-jkc
TITLE:

Fluralaner (Bravecto®) induces long-term mortality of Lutzomyia longipalpis after a blood meal in treated dogs

KEY WORDS:

Fluralaner; Dogs; Lutzomyia longipalpis; Leishmania infantum; Spectroscopy; Chemometrics; Multivariate analysis.

PAGES: 136
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Visceral leishmaniasis is a serious and chronic disease with a geographic spread in

Brazil due to the absence of a treatment that eliminates the parasite, ineffective control

measures, and diagnostic methods with low accuracy. The study aimed to evaluate

the systemic insecticidal activity of a single oral dose of fluralaner against Lutzomyia

longipalpis, as well as evaluate the mid-infrared as an alternative for Leishmania

infantum detection in canine serum samples. Mongrel dogs were recruited and

randomized into two groups, fluralaner (n = 4) and control (n = 4), and female

specimens of Lu. longipalpis were allowed to feed on all dogs before treatment, on day

one post-treatment, and then monthly until 1-year post-treatment. In addition,

attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR)

combined with multivariate analysis was employed to distinguish samples from dogs

infected with L. infantum in different clinical forms (n = 29) from healthy animals (n =

30). After data analysis, there was 100% mortality of Lu. longipalpis for up to five

months after treatment initiation. The efficacy of fluralaner ranged from 100% on day

1 to 68% at six months with a decrease to 1.4% at one-year post-treatment. ATR-FTIR

was efficient in differentiating samples from infected and uninfected dogs with 100%

sensitivity and specificity using the successive projections algorithm with linear

discriminant analysis (SPA-LDA), SPA with support vector machine (SPA-SVM), and

the genetic algorithm with LDA (GA-LDA). These satisfactory results suggest that

fluralaner may be used as a control strategy for VL in endemic areas, as well as

spectroscopy associated with multivariate analysis demonstrates its potential as an

alternative tool for the VL diagnosis in dogs.

COMMITTEE MEMBERS:
Externa ao Programa - 093.077.257-13 - JOANNA GARDEL VALVERDE GALVAO
Externo à Instituição - LEOMIR AIRES SILVA DE LIMA - Estácio
Presidente - 1752367 - PAULO MARCOS DA MATTA GUEDES
Externo ao Programa - 2213126 - VALTER FERREIRA DE ANDRADE NETO - nullExterno à Instituição - VERUSKA CAVALCANTI BARROS - UFPI