Banca de QUALIFICAÇÃO: KYVIA REGINA FERNANDES VARELA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : KYVIA REGINA FERNANDES VARELA
DATE: 29/10/2022
TIME: 14:00
LOCAL: Sessão de videoconferência: https://meet.google.com/yct-adqp-nzt
TITLE:

FORMULAÇÃO DE PROTÓTIPOS DE VACINAS DE DNA E AVALIAÇÃO DE PARÂMETROS FÍSICO-QUÍMICOS E BIOLÓGICOS

KEY WORDS:

Plasmids. DNA vaccines. Polymeric nanoparticles. Polyethyleneimine. Gene Transfer.

PAGES: 95
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

DNA vaccines, also called third-generation vaccines or genetic vaccines, are based on the use of plasmids that encode pathogen gene sequences as an antigenic candidate for inducing immunity. These vaccines have numerous advantages when compared to other vaccines; are easy to develop, low cost of production, safety and induction of cellular and humoral immune response. However, its main disadvantage is a reduced rate of cell transfection and consequently low endogenous production of the antigen of interest. This disadvantage is mainly due to the degradation of plasmids by nucleases present in tissues and inside cells. In this scenario, developing a strategy to overcome the enzymatic digestion barrier of plasmids during the immunization process seems to be an important means to improve gene transfer. Thus, the study aims to develop a nanosystem containing plasmids used in DNA vaccine models. Specifically plasmids pVAX1, pVAX1lacZ and nTSApVAX1, incorporated into polymeric polylactic acid (PLA) nanoparticles, with and without the presence of polyethyleneimine (PEI), which can protect and increase the levels of gene transfer of the plasmids, thus, building eficiente vaccine prototypes. The parameters of the systems that have been evaluated so far, based on the average diameter of the particles, were the polydispersity index (PDI) and zeta potential, in addition to performance assays, such as: physical-chemical stability, electrophoretic mobility delay, protection against enzymatic degradation for different sources and types of enzymes, and in vitro cell viability. The nanoformulations produced had an average size around 250 nm, PDI <0.3. The cationic nanoformulations (PLA + PEI + pDNA) showed good complexation between polymers and pDNA, as there was no appearance of bands in the electrophoresis assays, even against the degradation enzymes, also suggesting protection. The same profile was not observed with the anionic nanoformulations (PLA + pDNA), indicating a not so efficient binding between the components. All systems produced showed physicochemical stability, as well as adequate cell viability. Thus, the nanoformulated plasmids in this study prove to be promising carriers in the development of vaccine prototypes and DNA vaccines, making it desirable to carry out further studies in vitro and in vivo.

COMMITTEE MEMBERS:
Presidente - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Externa ao Programa - 2569445 - LOURENA MAFRA VERISSIMO - nullExterna à Instituição - WENDY MARINA TOSCANO QUEIROZ DE MEDEIROS - IFRN