Banca de DEFESA: SAULO VICTOR E SILVA
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : SAULO VICTOR E SILVA
DATE: 12/05/2022
TIME: 08:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/ubk-pzff-eny
TITLE:
PROPRIEDADES ANTIOXIDANTE DA COENZIMA Q10 INDUZIDA PELO ZINCO EM INDIVÍDUOS COM SÍNDROME DE DOWN
KEY WORDS:
Oxidative stress, Down Syndrome, antioxidants, coenzyme Q10, zinc.
PAGES: 186
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
Down Syndrome (DS) is known for the trisomy of chromosome 21, characterized by
several physiological and metabolic alterations, among them the presence of oxidative
stress as a result of the imbalance between the amount of reactive oxygen species (ROS)
and antioxidant enzymes. Compounds with antioxidant properties such as coenzyme
Q10 (CoQ10) and zinc (Zn) may reduce the cellular imbalances caused by the increase
of ROS. Thus, the present study aimed to evaluate the antioxidant potential of CoQ10
combined with Zn in individuals with DS. Initially, an in vitro study was performed to
evaluate the antioxidant potential of CoQ10, Zn, and their combination in a model of
oxidative stress in CHO-K1 cells induced by Arsenic (As). The determination of cell
viability for each treatment was done by crystal violet, MTT and Alamar Blue®
colorimetric assays. The antioxidant activity by DPPH radical reduction assays, ferric
reducing power (FRAP), aqueous hydroperoxide formation and ROS determination. In
parallel, a clinical, double-blind, randomized study was performed in children with DS
aged 2 to 9 years to evaluate the effect of CoQ10 supplementation at 4 mg/kg/day, Zn at
11 mg/day and CoQ10+Zn. Biochemical parameters such as glycemic control, lipid
profile and renal function, food consumption, anthropometric parameters, lipid
peroxidation, antioxidant potential by superoxide dismutase (SOD) and glutathione
peroxidase (GPx) enzymatic activity, and non-enzymatic by glutathione reductase
(GSH), and serum Zn profile were evaluated. In the in vitro study, cell viability assays
showed that both CoQ10 and its combination with Zn showed cytoprotective effect
independent of the concentrations analyzed, unlike cells treated only with Zn when
exposed to cell stress situations. CoQ10 showed antioxidant activity, independent of the
concentration used, but when combined with Zn it did not show any increase in its
activity at the cellular level, while Zn in high concentrations showed a pro-oxidant
effect causing cellular damage. In the in vivo study, the individuals presented serum Zn
concentration below the recommended level, thus, Zn supplementation in combination
with CoQ10 could add nutritional value to the antioxidant activity of CoQ10, presenting
satisfactory results to the supplementation of these micronutrients. Thus, the study
observed a greater deficiency of Zn in children with DS and that the supplementation
with CoQ10, when combined with Zn, unlike what was observed in the in vitro study, can present an increase in antioxidant activity, besides reducing lipid peroxidation and
ensuring the preservation of antioxidant enzymes.
BANKING MEMBERS:
Presidente - 346847 - MARIA DAS GRACAS ALMEIDA THORNTON
Interna - 061.474.624-82 - KARLA SIMONE COSTA DE SOUZA - UFRN
Interno - 2275890 - MARCELO DE SOUSA DA SILVA
Externo à Instituição - JULIANA PADILHA RAMOS NEVES - UNINASSAU
Externo à Instituição - GABRIEL ARAÚJO DA SILVA - UEAP