Banca de DEFESA: LUANA MEIRINHO DECOSIMO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUANA MEIRINHO DECOSIMO
DATE: 21/02/2022
TIME: 09:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/hko-ibnv-gcv
TITLE:

Zebrafish as an in vivo model: Applications and uses

KEY WORDS:

Alzheimer's disease, Erythrina velutina, alkaloids, zebrafish.

PAGES: 88
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Alzheimer’s disease (AD) is characterized by a neurodegenerative disorder that causes irreversible memory loss, being the main cause of dementia in the elderly population. The pathology is not fully understood but presents as main causes some metabolic changes that result in the characteristic signs of the disease, including deposition of beta-amyloid protein plaques and formation of neurofibrillary tangles in the hippocampus region and cerebral cortex, as well as sympathetic dysfunction with reduced cholinergic activity. Currently, the therapeutic arsenal of this disease is limited to some substances with action in the inhibition of the enzyme cholinesterase that blocks the effects of acetylcholine reduction, resulting in a neuroprotective effect. This therapy has only the ability to improve the quality of life, but does not prevent the progression of the disease. Therefore, new natural product options become interesting. Due to the great biodiversity of medicinal plants found in Brazil, mainly in the biome of the Caatinga exclusive to the Brazilian territory. One of these plants with bioactive potential is Erythrina velutina, which has a popular therapeutic use and has in its composition isoquinolinic alkaloids that have demonstrated psychoactive activities. Thus, the objective of the study was to evaluate the in vivo biological potential of fractions enriched in E. velutina seed alkaloids using zebrafish (Danio rerio) as an experimental model. The seeds were crushed and subjected to hydroethanolic extraction by static maceration, after filtration and drying of the extract, an acid-base extraction was performed to obtain the fraction enriched in alkaloids. A chromatogram with two main peaks was obtained by gas chromatography, Both peaks were analyzed by mass spectrometry where peak I with retention time at 28,293 min corresponds to the erythsodine alkaloid and peak II with retention time at 31,682 min corresponds to the erythrocyte alkaloid. This sample was used in zebrafish biological tests for toxicological evaluation in embryos and adults. The fraction has a lethal concentration for embryos at 17.40ug/ml, but is not toxic to adults. In adults, the fraction caused a response of decrease to anxiety-type behavior. In short-term memory tests, treatment with the fraction improved the performance of the animals, but no action of the long-term memory fraction was observed.

BANKING MEMBERS:
Externa ao Programa - 1644341 - ANA CAROLINA LUCHIARI
Presidente - 1492900 - CICERO FLAVIO SOARES ARAGAO
Externo ao Programa - 811.318.790-15 - FABIANO PERES MENEZES - UFRN
Externa à Instituição - PERCILIA CARDOSO GIAQUINTO - UNESP