Banca de QUALIFICAÇÃO: SAULO VICTOR E SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : SAULO VICTOR E SILVA
DATE: 20/07/2020
TIME: 13:00
LOCAL: Videoconferência
TITLE:

Coenzyme Q10 can prevent the cytotoxic effect of zinc and arsenic

KEY WORDS:

Oxidative stress, Down Syndrome, antioxidants, ubiquinol, zinc.

PAGES: 170
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Oxidative stress is described as the imbalance between the amount of reactive oxygen species (ROS) and antioxidant enzymes causing cell damage and consequent premature aging in individuals with Down Syndrome (DS). Compounds with antioxidant properties such as coenzyme Q10 (CoQ10) and trace elements such as zinc (Zn) can reduce cellular imbalances caused by the increase in ROS. Thus, the present study aimed to evaluate the antioxidant response of CoQ10 induced by Zn in individuals with Down Syndrome. Initially, we conducted a study with children with DS analyzing the methods for assessing the antioxidant potential by determining lipid peroxidation (TBA concentrations), enzymatic activity (SOD and GPx), non-enzymatic (GSH) and serum CoQ10 and Zn dosage. . In parallel, we conducted an in vitro study on a model of oxidative stress in CHO-K1 cells induced by Arsenic (As) to determine cell viability in different concentrations for each treatment by colorimetric crystal violet, MTT and Alamar Blue® assays, we measure the antioxidant activity by the DPPH radical reduction assays, and by the ferric reducing power (FRAP), and the conditions for maximum stress oxidation without cytotoxic effect were established by the formation of aqueous hydroperoxide. Cell viability assays showed that both CoQ10 and its combination with Zn have a cytoprotective effect regardless of the concentrations analyzed, unlike cells treated with Zn alone when exposed to situations of cell stress. CoQ10 showed potent antioxidant activity, regardless of the concentration used, but did not show any increase in its activity at the cellular level when combined with Zn, which, when used in higher concentrations, showed a pro-oxidant effect causing cell damage. The early stimulus to a healthy diet associated with the use of supplementation with antioxidant compounds such as CoQ10 and Zn can prevent various changes with the increase of oxidative stress in DS.

BANKING MEMBERS:
Externa ao Programa - 1640129 - ADRIANA DA SILVA BRITO
Externo à Instituição - ALEXANDRE COELHO SERQUIZ - UNI-RN
Presidente - 2275890 - MARCELO DE SOUSA DA SILVA