Banca de DEFESA: LETÍCIA GONDIM LAMBERT MOREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LETÍCIA GONDIM LAMBERT MOREIRA
DATE: 28/02/2020
TIME: 09:00
LOCAL: SALA DE VIDEOCONFERÊNCIA DO IMD / UFRN
TITLE:

Tropane alkaloid of Erythroxylum pungens: Isolation, in silico assay and the bioactive potential evaluation in zebrafish

KEY WORDS:

Erythroxylum pungens; Erythroxylaceae, Tropane alkaloids; Danio rerio; bioprospection.

PAGES: 93
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Brazilian biodiversity offers an enormous wealth of flora, a unique opportunity for bioprospecting activity. Among the bioactive molecules found in most plant organisms, alkaloids represent a wide variety of chemical structures. Among the Brazilian biomes, the Caatinga, predominant vegetation in the Northeast region, offers biological heritage that cannot be found anywhere else on the planet. It is in this xeric environment that Erythroxylum pungens O.E Shulz is found, a tree-shrub species with great potential in little explored bioactive alkaloids. The objective of this work is the bioprospecting of tropane alkaloids of Erythroxylum pungens OE Shulz, collected in a natural environment, the evaluation of the effect of water stress on metabolism under young plants and the evaluation of the bioactive potential in vivo in a Danio rerio (Zebrafish) model organism, as well as, in silico analysis of the major alkaloid. In Chapter 1, the water stress experiment was carried out by suspending irrigation in specimens of E. Pungens in up to 20 days. We conclude that E. Pungens is able to store high levels of proline, osmoregulatory and protective amino acid, in response to drought tolerance. Also, from analysis by CG-MS, it was possible to observe that the production of tropane alkaloids is more related to the development phase than to the water stress condition in which the plant was subjected. In chapter 2 was identified by means of GC-MS spectroscopy analysis were 13 tropane alkaloids identified in this study, with three of these species and to unpublished alkaloid class. Regarding toxicity, several teratogenic effects were observed at 5mg/L and the lethal dose for zebrafish embryo was established at 4.73 mg/L. No mortality was observed in adults, but a behavioral screen showed psychostimulatory action at 30 mg/L. In silico, targets involved in the antidepressant pathways were identified by docking with the alkaloid. Overall, E. pungens is a source of unique tropane alkaloids, as the 3α-(2-methylbutyryloxy)tropan-6,7-diol, which seems to be involved in zebrafish behavioral changes and deserve future investigation as a possible new molecule in the treatment of depressive-like symptom or hallucinogens effects.

BANKING MEMBERS:
Externo à Instituição - JEAN PAULO DE ANDRADE - UTalca
Externo à Instituição - FABIANO PERES MENEZES - UFRN
Externo ao Programa - 2276354 - LEANDRO DE SANTIS FERREIRA
Presidente - 1871916 - RAQUEL BRANDT GIORDANI