Banca de DEFESA: BRUNO AMORIM DO CARMO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : BRUNO AMORIM DO CARMO
DATA : 25/03/2019
HORA: 14:00
LOCAL: SALA 2 DO PPGCF
TÍTULO:

Potent and Broad-Spectrum Antimicrobial Activity of Analogs from the Scorpion Peptide Stigmurin

PALAVRAS-CHAVES:

Tityus stigmurus; analog peptides; scorpion peptides; antimicrobial agent; molecular dynamics; bacterial membrane.

PÁGINAS: 95
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Scorpion venom constitutes a rich source of biologically active compounds with high potential for therapeutic and biotechnological applications, which can be used as prototypes for the design of new drugs. The aim of this study was to characterize the structural conformation, to evaluate the antimicrobial activity and to give an insight about the possible action mechanism underlying it, using the molecular dynamics of two new analog peptides of the scorpion peptide Stigmurin, named StigA25 and StigA31. The amino acid substitutions in the native sequence for lysine residues resulted in peptides with higher positive net charge and hydrophobicity, with an increase in the theoretical helical content. StigA25 and StigA31 showed the capacity to modify their structural conformation according to the environment, and were stable to pH and temperature variation, results similar to the native peptide. Both analog peptides demonstrated broad-spectrum antimicrobial activity in vitro, showing an effect superior to that of the native peptide, being non-hemolytic at the biologically active concentrations and potential antiproliferative activity in HeLa cells. Therefore, this work demonstrates the therapeutic potential of analog peptides of Stigmurin and the promising effect of peptide engineering for the potential development of new therapeutic agents.

MEMBROS DA BANCA:
Externo ao Programa - 1959889 - DAVI SERRADELLA VIEIRA
Externo ao Programa - 1715230 - JOSELIO MARIA GALVAO DE ARAUJO
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externo à Instituição - RAFAEL MATOS XIMENES - UFPE