Banca de QUALIFICAÇÃO: MARIANA BORGES LOPES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARIANA BORGES LOPES
DATA : 28/09/2017
HORA: 08:30
LOCAL: Sala 01 - PPGCF
TÍTULO:

O PAPEL DA EXPRESSÃO DOS GENES PLA2G2A E HK2 E SEUS miRNAs REGULATÓRIOS NA FISIOPATOLOGIA DA CARDIOMIOPATIA DIABÉTICA

PALAVRAS-CHAVES:

Diabetes mellitus. Diabetic Cardiomyopathy. H9c2. Animal model. Gene Expression.

PÁGINAS: 91
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Diabetic cardiomyopathy is a chronic complication of diabetes that is associated with increased mortality and morbidity of individuals affected with this disease. However, it molecular basis is not fully elucidated. In this context, the aim of this study is to evaluate the expression of the PLA2G2A and HK2 genes and it regulatory miRNAs in vitro through model of H9c2 cells and in vivo using an experimental model of type 1 diabetes induced by streptozotocin. H9c2 cells, Rattus norvegicus myoblast lineage, growed in DMEM medium supplemented with 10% FBS at 37° C and 5% CO2. The cells were plated at a density of 1 x 10⁵ cells/mL in triplicate and incubated for 24 hours and treated under the following conditions: normoglycemic medium (NG, 5.5 mmol/L glucose), osmolarity control medium (CO, 5.5 mmol/L glucose and 19.5 mmol/L mannose solution) and hyperglycemic medium (HG, 25 mmol/L glucose). Cell viability was assessed by the MTT assay and the gene expression by RT-qPCR. Wistar rats had diabetes induced by injection of streptozotocin (40 mg/kg, IV). After a 30-day period, control animals (n = 7) and diabetic animals (n = 7) were euthanized and the left ventricle was used for the analysis of gene expression by RT-qPCR. In agreement with an in silico study previously performed by our group, PLA2G2A gene expression was increased about 3-fold in the HG group of the in vitro study (p = 0.043), as well as increased in the group of diabetic rats when compared to their respective controls and (p = 0.004). In addition, expression of the HK2 gene, which was reduced in our in silico study, showed a downward trend in the HG group of the in vitro study (p ≥ 0.05) and was significantly decreased in the group of diabetic rats (p = 0.010) when compared to their respective controls. The experimental data performed in this study confirm an in silico approach used prior, as well as, affirm the relevant role of the analyzed genes in the pathophysiology of diabetic cardiomyopathy.

MEMBROS DA BANCA:
Presidente - 2323511 - ADRIANA AUGUSTO DE REZENDE
Externo ao Programa - 1667882 - BENTO JOAO DA GRACA AZEVEDO ABREU
Externo ao Programa - 1199127 - SILVIA REGINA BATISTUZZO DE MEDEIROS