Banca de DEFESA: SARA RAYANA MORAIS FERNANDES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : SARA RAYANA MORAIS FERNANDES
DATA : 28/07/2016
HORA: 09:00
LOCAL: Banca Fechada ao Público
TÍTULO:

DESENVOLVIMENTO DE HIDROGÉIS DE QUITOSANA ASSOCIADA A OUTROS
BIOPOLÍMEROS PARA VEICULAÇÃO E LIBERAÇÃO DA ENZIMA PAPAÍNA

PALAVRAS-CHAVES:

Hydrogels.Papain.Chitosan.Guar Gum. Hydroxyethylcellulose.

PÁGINAS: 129
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

The papain is an enzyme extracted from Carica papaya that has selective chemical debriding, antiinflammatory and cicatrizing properties with important therapeutic application on burns. The use of polymeric biomaterials for organs and tissues regeneration has been widespread on the scientific literature and commercially available products. The development of hydrogels based on biopolymers containing papain presents the advantage of to may associate two mechanisms to treat burns, an autolytic (biopolymers with hydratation of the burn) and an enzymatic (papain with chemical debridement). The hydrogels were developed by physical blend of the chitosan and other biopolymers (cellulose or gums derivated ones) at different concentrations to obtain the characteristics of a topical formulation. The hydrogels that best meets these requirements were ones of chitosan 3% with guar gum 3 %, and chitosan 3% with hydroxyethylcellulose 3 %. It was necessary the chemical crosslinking of these hydrogels with vanillin, and further incorporation of papain enzyme on the concentrations of 1 % and 2 %. The hydrogels were characterized by evaluation of swelling, gel strength, rheology, pH, macroscopic aspects, syneresis area and in vitro release kinetics. The presence of papain was able to decrease the swelling capability and strength of hydrogels. While that chemical crosslinking with vanillin at most hydrogels did not affect the swelling capability and increased strength of the gels. The formulations had pH values compatible with at the skin, ranging between 4.7 and 5.3. On rheology, all hydrogels exhibited pseudoplastic behavior, with higher apparent viscosity values for the chitosan with guar gum hydrogels in relation to the hydroxyethyl cellulose hydrogels and, in particular, for crosslinked ones with vanillin and at absence of papain. They were not observed differences among the hydrogels in the syneresis area test and were they found analytical selectivity problems in the quantification of enzyme activity. In the in vitro release kinetics, the protein measurement of papain was performed by BCA method and it was analytically suitable only for the chitosan hydrogels with hydroxyethylcellulose and without vanillin. An increase in the burst effect was observed with the increase of incorporated papain and the diffusion is involved in release mechanism of papain from the hydrogels. Hydrogels of biopolymers chitosan with guar gum, and of hydroxyethylcellulose with chitosan containing papain were obtained with success and physico-chemically characterized so to complay with the requirements of topical product applicable to improve the functionality of this enzyme in the treatment of burns.

MEMBROS DA BANCA:
Externo à Instituição - ELISANGELA AFONSO DE MOURA MENDONÇA - UFPB
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Interno - 6330567 - TULIO FLAVIO ACCIOLY DE LIMA E MOURA