Banca de DEFESA: MARIANA CARVALHO XEREZ

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARIANA CARVALHO XEREZ
DATA : 26/02/2018
HORA: 08:30
LOCAL: AUDITÓRIO DO DEPARTAMENTO DE ODONTOLOGIA DA UFRN
TÍTULO:

CLIN4 AND α-SMA IMMUNO-EXPRESSION IN ORAL SCAMMAL CARCINOMAS AND VERRUCOSAL ORAL CARCINOMAS

 

PALAVRAS-CHAVES:

Squamous Cell Carcinoma; Carcinoma Verrucoso; Myofibroblasts

PÁGINAS: 92
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:

Introduction: Among the malignant neoplasias affecting the oral cavity, oral squamous cell carcinoma (OSCC) is the most frequent presenting high rates of morbidity and mortality, and oral verrucous carcinoma (OVC), which exhibits a behavior distinct from a low grade variant of oral squamous cell carcinoma. The development and progression of these malignant neoplasms are related to the imbalance in the regulation of cell division and death associated to the tumor microenvironment. CLIC4 protein is related to the regulation of the cell cycle, being supraregulated in response to apoptosis and also participating in the process of transdifferentiation of fibroblasts in cancer-associated fibroblasts (CAF), which now express α-SMA. CAFs are the main cellular components of the tumor microenvironment, having been related to the aggressiveness and prognosis of several tumors. Materials and Methods: Twenty cases of OSCC and 15 cases of OVC were selected. From the medical records, clinical data regarding the age, gender and location of the lesion were collected. A morphological analysis was performed on HE and semiquantitative immunohistochemical expression of the CLIC4 and α-SMA proteins in samples of paraffin-shaped lesion material. Pearson's Chi-square test and Fisher's exact test were used to verify associations. The Spearman test was used to search for correlation. Significance was set at of 5%. Results: Among the OSCC, the majority occurred in male patients (n = 11; 55.0%), with a mean age of 66.95 years and the most affected anatomic location was tongue (n = 9; 45.0%). The OVC was the female sex (n = 9; 60.0%), the mean age was 61.71 years, and the alveolar ridge was the most affected (n = 4; 26.7%) and lip mucosa (n = 4; 26.7%). For the analysis of CLIC4 expression, its cellular location was considered. There was a decrease in nuclear labeling and increased cytoplasmic expression of CLIC4 in both OSCC and OVC cases. Comparing the lesions for CLIC4 labeling in the nucleus, cytoplasm and nucleus /cytoplasm of tumor epithelial cells, no significant difference was observed. When comparing CLIC4 expression in the OSCC and OVC stroma, a significant difference was observed (p <0.0001). The analysis of α-SMA immunostaining in mesenchymal cells of OSCC and OVC revealed an increase in the expression of this protein in the tumor stroma of both tumors, and no significant difference was observed between the lesions. When comparing the immunoexpression of αSMA and CLIC4 in the stroma of OSCC and OVC, a positive correlation was observed but not significant in OSCC (r: 0.350 and p: 0.130). In the OVC, a positive and significant correlation was observed between the CLIC4 and α-SMA proteins (r: 0.612 and p: 0.015). Conclusions: Decreased nuclear marking and increased cytoplasmic expression of CLIC4, associated with increased expression of this protein in the tumor stroma, seems to contribute to the process of carcinogenesis and progression of OSCC and OVC and may have an influence on α-SMA expression.

MEMBROS DA BANCA:
Presidente - 1258707 - ANTONIO DE LISBOA LOPES COSTA
Interno - 350485 - HEBEL CAVALCANTI GALVAO
Externo à Instituição - MARIA DE LOURDES SILVA DE ARRUDA MORAIS - UERN