Banca de DEFESA: ROMULO AUGUSTO DE PAIVA MACEDO
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : ROMULO AUGUSTO DE PAIVA MACEDO
DATA : 23/02/2018
HORA: 14:30
LOCAL: SALA VII DO DEPARTAMENTO DE ODONTOLOGIA
TÍTULO:
EFFECT OF PHOTOBIOMODULATION ON THE PROLIFERATION OF STEM CELLS FROM HUMAN EXFOLIATED DECIDUOUS TEETH CULTURED ON POLYLACTIC ACID FILMS
PALAVRAS-CHAVES:
Stem cells; Polylactic acid; Photobiomodulation; Cell proliferation
PÁGINAS: 84
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:
Photobiomodulation (PBM) stimulates the proliferation of different cell types, including stem cells. Stem cells from human exfoliated deciduous teeth (SHEDs) present a greater differentiation potential compared to other mesenchymal stem cells, being the focus of several studies in the field of tissue engineering. In order for cells to proliferate and differentiate in vitro it is necessary to develop a favorable microenvironment, which can be provided by a biomaterial that mimics the natural extracellular matrix, with polylactic acid (PLA) being distinguished for this purpose due to its properties of biocompatibility and biodegradability, as well as low cost. The aim of this study was to evaluate the effect of PBM in different doses on the proliferation and viability of SHEDs cultured on two-dimensional PLA scaffolds. SHEDs were cultured on the films and divided into three groups: (C) non-irradiated control; (L1) irradiated with a dose of 1 J/cm2; and (L4) irradiated with a dose of 4 J/cm2. The irradiations were performed with an InGaAlP diode laser, with wavelength of 660 nm and power of 30 Mw, in a single dose. Cell viability and proliferation were evaluated at 24, 48 and 72 h after irradiation by Trypan blue exclusion method and Alamar blue assay, while cell morphology and distribution on the surface of the films were evaluated by SEM at 72 hours. The quantitative data were submitted to non-parametric statistical tests. The results of the cell counts by Trypan blue exclusion method revealed that groups L1 and L4 presented a higher number of viable cells compared to the C group at all the intervals, with statistically significant differences between L1 and C at 48 and 72 h (p<0.01) and between L4 and C at 24 (p<0.05), 48 (p<0.0001) and 72h (p<0.01). Data from Alamar blue assay confirmed that groups L1 and L4 exhibited greater cell proliferation compared to group C, with significant differences between L1 and C at 48 and 72 h (p<0.0001) and between L4 and C in 24 (p<0.01), 48 (p<0.001) and 72 h (p<0.0001). The percentage of reduction of Alamar blue was significantly higher in L4 compared to L1 only at 24 h (p<0.05). Analysis of the samples by SEM showed that in the irradiated groups the cells had a more homogeneous distribution along the surface of the films and a higher cell density compared to the group C, with this difference being even more evident in the group L4. We conclude that PBM in the studied parameters, especially with the dose of 4 J/cm2, stimulates the proliferation of SHEDs in contact with PLA films, thus constituting a methodological tool with potential application in tissue engineering techniques.
MEMBROS DA BANCA:
Presidente - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Externo à Instituição - CARLOS EDUARDO BEZERRA DE MOURA - UFERSA
Interno - 1298808 - MARCIA CRISTINA DA COSTA MIGUEL