Banca de DEFESA: LARISSA SANTOS AMARAL ROLIM

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LARISSA SANTOS AMARAL ROLIM
DATA : 23/02/2018
HORA: 14:30
LOCAL: AUDITÓRIO DO DEPARTAMENTO DE ODONTOLOGIA DA UFRN
TÍTULO:

COMPARATIVE ANALYSIS OF TWIST AND PODOPLANIN IMUNOEXPRESSION BETWEEN ORAL TONGUE AND LOWER LIP SQUAMOUS CELL CARCINOMA.

PALAVRAS-CHAVES:

Carcinoma, Squamous Cell; Twist-Related Protein 1; Epithelial-Mesenchymal Transition; Pathology Oral

PÁGINAS: 100
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:

Introduction: Oral cancer is a major public health problem worldwide, and among oral cancers, Oral Squamous Cell Carcinoma (OSCC) is the most common. It is believed that the mechanism responsible for tumor progression is based on collective invasion of cell groups or individual cells, called Epithelial-Mesenchymal Transition (EMT), where there is a decrease in the expression of epithelial biomarkers and increased expression of mesenchymal biomarkers as Twist. Podoplanin (PDPN), a transmembrane glycoprotein, is related to cellular contractile properties and reorganization of the cytoskeleton and is assumed to be involved in the motility of neoplastic cells that are undergoing through EMT to guide the cell complex during an invasion. Objective: The aim of this study was to perform a comparative analysis of the immunoexpression of PDPN and Twist among 40 cases of lower lip CCEs and 36 cases of oral tongue, (primary tumor size, regional and distal lymph node metastasis, clinical staging, histological grade of malignancy and histological pattern of invasion). Methods: To evaluate the histopathological grade of malignancy, the system proposed by Brandwein-Gensler et al. (2005) was used. For the two markers, five types of immunohistochemical analysis were performed: analysis of the invasion front, tumor compressive areas, large tumor islands (> 15 neoplastic cells) and nests/dissociated cells (<15 neoplastic cells). The non-parametric Mann-Whitney test was used for the analysis of the relationships between the clinical and histopathological parameters and the PDPN and Twist immunoexpressions. To verify possible correlations between the PDPN and Twist immunoexpressions, the Spearman (r) correlation test was performed. For all statistical tests, a significance level of 5% (p <0.05) was established. Results: No statistically significant differences were found between PDPN and Twist immunoexpression and tumor size, metastasis, and clinical staging on the invasive front of lower lip SCC (p > 0.05). However, statistically significant differences were found between the cytoplasmic immunoexpression of PDPN and the invasion patterns type 4 and 5 (p = 0.032) of Brandwein-Gensler et al. (2005). Also, no statistically significant differences were found between PDPN and Twist immunoexpression and tumor size, metastasis and clinical staging on the invasive front of oral tongue SCC (p > 0.05). Statistically significant differences were observed between the cytoplasmic (p = 0.006), membrane (p = 0.030) and general (p = 0.025) PDPN expression in oral tongue SCC with the invasion patterns type 4 and 5 of Brandwein-Gensler et al. (2005). In addition, the Spearman correlation test demonstrated statistically significant negative correlations between PDPN membrane expression and Twist general expression (r = -0.356, p = 0.024) and Twist cytoplasmic expression (r = -0.336; p = 0.034) in the lower lip SCCs. Conclusions: It is concluded that PDPN expression is inversely related to Twist in lower lip SCC, it was demonstrated that both proteins are associatved with a worse invasion pattern in SCC. It has also been hypothesized that the relationship of PDPN with Twist in OSCC may be more involved with a partial transition from the epithelial to the mesenchymal phenotype than a complete transition coordinated by PDPN.

MEMBROS DA BANCA:
Externo à Instituição - CASSIANO FRANCISCO WEEGE NONAKA - UEPB
Presidente - 344668 - LEAO PEREIRA PINTO
Interno - 346077 - LELIA BATISTA DE SOUZA