Banca de DEFESA: JULIANA CAMPOS PINHEIRO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JULIANA CAMPOS PINHEIRO
DATA : 27/02/2018
HORA: 14:30
LOCAL: AUDITÓRIO DO DEPARTAMENTO DE ODONTOLOGIA DA UFRN
TÍTULO:

 

RELATIONSHIP BETWEEN MAST CELLS AND E-CADHERIN IN ODONTOGENIC KERATOCYSTS AND RADICULAR CYSTS.

PALAVRAS-CHAVES:

Odontogenic keratocyst, Radicular cyst, Mast cells, E-cadherin.

PÁGINAS: 89
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:

Mast cells are secretory cells that release different chemical mediators, among them tryptase, participating in both physiological and pathological stimuli. E-cadherin is an element of the cadherin family known to play an important role in the regulation of intercellular adhesion in epithelial tissues. The present study aimed to evaluate the relationship between mast cells and E-cadherin expression in odontogenic keratocysts and root cysts. The immunohistochemical evaluation of tryptase consisted of the quantification of degranulated and non-degranulated mast cells in 15 fields, 5 in the epithelial tissue, 5 in the superficial connective tissue and 5 fields in the deep connective tissue. The evaluation of E-cadherin was performed semi-quantitatively, using the scores: 1 (0 to 50%) and 2 (> 50%), also observing the cellular immunoreactivity pattern (membrane, cytoplasmic, membrane and cytoplasmic) in addition to the verification of the size of the lesions from the data collection in the clinical records of the patients. The analysis of the cystic lesions studied revealed a higher total mean number of mast cells in the root cysts when compared to odontogenic keratocysts (95% CIs). With regard to the amount of mast cells in the epithelial component, a higher median was observed in the root cysts in relation to odontogenic keratocysts; in the connective tissue, odontogenic keratocysts were the largest median. When verifying the density of degranulated and non-degranulated mast cells through the Kruskal-Wallis non-parametric test, it was found that the highest concentrations were related to degranulated mast cells in the epithelium of root cysts (KW = 30,343; p = 0.000). Odontogenic keratocysts the highest densities of these degranulated cell types were found in connective tissue (KW = 0.092; p = 0.762). When examining the expression of E-cadherin in the epithelial component, a similarity was observed between cystic lesions with greater expression in odontogenic keratocysts (p = 0.798), which presented membrane marking in 63.3% of the cases. Spearman's correlation analysis showed a negative correlation between E-cadherin expression and total mast cells (r = -0.311; p = 0.016), number of degranulated mast cells (r = -0.255; p = 0.049) and the location of these cell types in total and deep connective tissue in both root cysts and odontogenic keratocysts. There was also another negative correlation between intraepithelial mast cells (r = -0.265; p = 0.016) and cystic lesion size. In view of these findings, we observed that the higher concentration of mast cells in the root cysts confirms their inflammatory nature, highlighting the greater amount of degranulated mast cells in the epithelial component, reflecting less e-cadherin expression when compared to odontogenic keratocysts. Possible tryptase activity in the alteration of adhesion joints and consequent increase in epithelial permeability. Regarding the size of the lesions, it was verified that the higher mast cell densities in the epithelial component were identified in smaller lesions, suggesting an action of these cell types in an initial stage of growth of cystic lesions studied.

MEMBROS DA BANCA:
Externo à Instituição - CYNTIA HELENA PEREIRA DE CARVALHO - UFCG
Interno - 346077 - LELIA BATISTA DE SOUZA
Presidente - 2859541 - PEDRO PAULO DE ANDRADE SANTOS