Banca de DEFESA: GLÓRIA MARIA DE FRANÇA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : GLÓRIA MARIA DE FRANÇA
DATA : 23/02/2018
HORA: 14:30
LOCAL: SALA III DO DEPARTAMENTO DE ODONTOLOGIA - UFRN
TÍTULO:

IMMUNOHISTOCHEMICAL STUDY OF MACROPHAGES SUBPOPULATIONS IN RADICULAR CYST AND PERIAPICAL GRANULOMAS

PALAVRAS-CHAVES:

Radicular Cyst; Periapical Granuloma; Macrophages.

PÁGINAS: 61
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:

Introduction: Radicular cysts (RCs) and periapical granulomas (PGs) develop in response to inflammatory mediators from pulp necrosis. Macrophages play an important role in the etiopathogenesis of these lesions and are involved in the protective response of the host as well as in the perpetuation of inflammatory reactions. The infiltration of macrophages into sites of chronic inflammation allows the phagocytosis of foreign agents, the release of chemical mediators and may also act as antigen-presenting cells. Aim: to identify the subpopulations of macrophages M1/M2 in RCs and PGs, additionally relates them to clinical and morphological aspects. Material and Methods: An immunohistochemical analysis was performed on 30 RCs and 30 PGs. The M1 macrophages subpopulation was evaluated by the percentages of CD68 immunostaining associated with the TNF-α inflammatory cytokine and the M2 macrophages subpopulation by its specific CD163 antibody. Clinical, radiographic, symptomatology, treatment and morphological parameters of the lesions were collected and a statistical significance level of 95% was adopted for statistical analysis. Results: The ratio of CD68⁺/CD163⁺ cells was higher in the RCs (median=1.22, p=0.002), and the highest TNF-α immunostaining scores were found in the RCs (p=0.018) hence the M1 phenotype polarization was viwed; in the PGs, the CD68⁺/CD163⁺ ratio was lower (median=1.02) and associated with a greater CD163⁺ immunostaing (p<0.001) hence the M2 phenotype polarization was viwed. In addition, CD68⁺ cells had higher percentages of immunostaining in RCs with smaller sizes (p=0.034). Conclusion: The increased CD68 immunostaining associated with TNF-α cytokine in RCs suggests the greatest differentiation for a M1 phenotype. The greater CD163 immunostaining in PGs provide greater differentiation for the M2 phenotype. Therefore, the inflammatory state promoted by the M1 macrophages is related to appearance and maintenance of RCs, on the other hand, the immunomodulatory state of the M2 macrophages is related to the development of PGs.

MEMBROS DA BANCA:
Externo à Instituição - ANA LUIZA DIAS LEITE DE ANDRADE - FACENE
Presidente - 350485 - HEBEL CAVALCANTI GALVAO
Interno - 2859541 - PEDRO PAULO DE ANDRADE SANTOS