Banca de DEFESA: YAILIT DEL CARMEN MARTINEZ VARGAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : YAILIT DEL CARMEN MARTINEZ VARGAS
DATA : 15/02/2018
HORA: 14:30
LOCAL: AUDITÓRIO DO DEPARTAMENTO DE ODONTOLOGIA DA UFRN
TÍTULO:

 STUDY OF IMMUNOHISTOCHEMICAL EXPRESSION OF ING3 AND ING4 PROTEINS IN BENIGN EPITELIAL ODONTOGENIC LESIONS

PALAVRAS-CHAVES:

Benign epithelial odontogenic lesions; tumor suppressor gene; immunohistochemistry; ING3; ING4.

PÁGINAS: 66
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:

Odontogenic cysts and tumors represent a heterogeneous group of lesions originated from epithelial or ectomesenchymal tissues from the embryonic development of the dental organ, affecting only the gnathic bones. Considering that these epithelial lesions have a distinct biological behavior, they have become the subject of numerous investigations. INGs genes play an important role in cell cycle control, senescence, DNA replication and repair, cell proliferation and apoptosis in various neoplasms. The aim of this study was to comparatively evaluate the immunohistochemical expression of ING3 and ING4 proteins in a series of cases of benign epithelial odontogenic lesions. The sample consisted of 20 ameloblastomas (AM), 15 adenomatoid odontogenic tumors (AOT) and 20 odontogenic keratocysts (OK). The immunohistochemical staining was evaluated semiquantitatively on each case studied according to the percentage of positively immunostained epithelial cells, which ranged from 0 to 3. For statistical analysis were used the Kruskal-Wallis and Spearman tests (p <0.05). Analysis of ING3 expression was positive in the cytoplasm and nucleus of epithelial cells without statistically significant difference between group of lesions studied. Analysis of ING4 expression revealed positivity in the cytoplasm of epithelial cells of all lesions studied. The cytoplasmic immunostaining score for ING4 revealed a statistically significant difference between the groups of lesions studied, with a higher frequency in AOT (p <0.05). After pairing, there was a significant difference between the AM and OK, AM and AOT (p <0.05). And the analysis of ING4 nuclear expression in the studied lesions revealed loss of nuclear expression, followed by reduced nuclear expression for cases of AM and OK, and positivity in the nucleus of AOT epithelial cells. The nuclear immunostaining score for ING4 revealed a statistically significant difference between the groups of lesions studied, with a higher frequency in AOT (p <0.05). After pairing, we observed a significant difference between AM and OK and between AM and AOT and between OK and AOT (p <0.05). In conclusion, the results of the present study suggest that the regulation of ING3 and ING4 in cell growth and apoptosis indicate that these proteins may play an important role in the development and progression of odontogenic lesions.

MEMBROS DA BANCA:
Interno - 347125 - ANA MIRYAM COSTA DE MEDEIROS
Externo à Instituição - DENISE HELEN IMACULADA PEREIRA DE OLIVEIRA - UFC
Presidente - 1258693 - LELIA MARIA GUEDES QUEIROZ