Banca de DEFESA: MARCELO ANDERSON BARBOSA NASCIMENTO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : MARCELO ANDERSON BARBOSA NASCIMENTO
DATA : 02/02/2018
HORA: 14:30
LOCAL: AUDITÓRIO DO DEPARTAMENTO DE ODONTOLOGIA DA UFRN
TÍTULO:

ANALYSIS OF SOX2, FGF-10 AND WNT-1 IMMUNOHYSOCHEMICAL EXPRESSION IN BENIGN EPITHELIUM ODONTOGENIC INJURIES

PALAVRAS-CHAVES:

Stem cells, Benign Odontogenic Epithelial Lesions; Immunohistochemistry; SOX2; FGF10; Wnt-1.

PÁGINAS: 97
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:

Dental development occurs from sequential interactions between the epithelium and the mesenchyme derived from the neural crest at different stages of histodifferentiation and morphodifferentiation. At the end of tooth development, the structures that participated in the formation of these tissues are expected to disappear or remain quiescent. It is not uncommon that the epithelial remnants of the tooth development originate lesions such as odontogenic cysts and tumors. In early tooth development, stem cell maintenance is regulated by specific transcription factors, which includes OCT-4, SOX-2, Nanog, Stat-3 and c-Myc and several other Homeobox genes and transcription pathways (SHH, Wnt/β-catenin, FGF, BMP) contribute to cell fate and differentiation. However, there is involvement of these genes and pathways in the pathogenesis of several types of tumors. The aim of the present study was to evaluate the immunoexpression of SOX2, FGF-10 and Wnt-1 in a case series of odontogenic lesions and some specimens of dental germs. The sample consisted of 20 Odontogenic Keratocysts (OK), 20 solid ameloblastomas (AM), 20 adenomatoid odontogenic tumors (AOT) and 10 calcifying epithelial odontogenic tumors (CEOT). Immunoexpression was evaluated according to the percentage of immunostained epithelial cells and intensity of the positive cells resulting in total immunostaining score (PIT) ranging from 0 to 7. The analysis of SOX2 immunoexpression revealed positivity in most cases of the lesions studied. The immunostaining score for SOX2 revealed a statistically significant difference between the groups of lesions studied, with a higher frequency in OK and CEOT (p < 0.001). After pairing, we observed a significant difference between AM and OK, AM and CEOT, OK and AOT, OK and CEOT, and AOT and CEOT (p <0.05). Analysis of the FGF-10 and Wnt-1 immunoexpression revealed positivity in all cases of the lesions studied, with no statistically significant difference between the groups of lesions studied (p = 0.628). There was a significant difference in relation to the positivity scores for Wnt-1 (p <0.001) with higher frequency in OK and AOT. After pairing, there was a statistically significant difference between AM and OK, AM and CEOT, OK and CEOT and, AOT and CEOT (p <0.05). The expression pattern of SOX2, FGF-10 and Wnt-1 in dental germs and odontogenic lesions evaluated here confirms the participation of these pathways in the tooth development as well as in the development of odontogenic lesions.

MEMBROS DA BANCA:
Externo à Instituição - BETANIA FACHETTI RIBEIRO - UNP
Interno - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Externo à Instituição - CASSIANO FRANCISCO WEEGE NONAKA - UEPB
Presidente - 346077 - LELIA BATISTA DE SOUZA
Interno - 1258693 - LELIA MARIA GUEDES QUEIROZ