Banca de DEFESA: TIAGO JOÃO DA SILVA FILHO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : TIAGO JOÃO DA SILVA FILHO
DATA : 02/02/2018
HORA: 08:30
LOCAL: AUDITÓRIO DO DEPARTAMENTO DE ODONTOLOGIA DA UFRN
TÍTULO:

EXTRACELLULAR VESICLES DERIVED FROM MACROPHAGES ALTER THE POTENTIAL OF INVASION, PROLIFERATION AND MIGRATION OF TONGUE SQUAMOUS CELLS CARCINOMA CELLULAR LINEAGES

PALAVRAS-CHAVES:

Tumor Microenvironment; Carcinoma, Squamous Cell; Cell Culture Techniques; Extracellular Vesicles.

PÁGINAS: 126
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:

Currently, cancer is considered as an entity composed of proliferating malignant cells associated with the different types surrounding cells, forming the tumor microenvironment (TME), where there is a constant exchange of information. One of the ways of communicating between different types of TME cells is through the release of extracellular vesicles (EVs), a field of study that remains poorly understood. The aim of the present study was to evaluate the effects of EVs released from TME macrophages, which are cells highly plastic in their phenotype (M1 showing an anti-tumor profile and M2 exhibiting a pro-tumor profile) in different cell lines of tongue squamous cells carcinoma (TSCC) regarding to invasive, proliferative and migratory capacity. It was observed that EVs samples obtained from macrophages were relatively pure in EVs, although they were non-specific subtypes. In the myoma invasion assay, it was observed that when inflammatory cells were co-cultured with HSC-3 cells, M1 cells inhibited invasion and M2 increased the invasive ability of the malignant cells. On the other hand, treatment with M1 EVs increased the invasive capacity of HSC-3 cells, and treatment with M2 EVs inhibited the invasion of these malignant cells, and a similar profile was observed in SCC-25 and SAS cells when they were submitted to the same treatments. When it was analyzed the proliferation of malignant cells in IncuCyte® treated with EVs derived from different types of macrophages at different concentrations, an increase in the proliferative ability of HSC-3 and SAS cells treated with M1 EVs was observed in a dose-dependent pattern. An increase in proliferative ability in dose-dependent profile was also observed when SAS cells were treated with M2 EVs. In the other proliferation assays performed in IncuCyte®, effects on proliferative capacity were also highlighted, however a dose-dependent pattern was not observed. In the IncuCyte® migration assay, significant differences were observed in the migration capacity of SCC-25 and SAS cells treated with different types of EVs at different concentrations when compared to the negative control. The findings of this study consolidate macrophages-derived EVs as pivotal factors in TSCC tumorigenesis, as well as permits discussions on the different effects of inflammatory cells on TME depending on the type of cell communication performed.

MEMBROS DA BANCA:
Interno - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Externo à Instituição - CASSIANO FRANCISCO WEEGE NONAKA - UEPB
Externo à Instituição - DENISE HELEN IMACULADA PEREIRA DE OLIVEIRA - UFRN
Interno - 1258693 - LELIA MARIA GUEDES QUEIROZ
Interno - 1298808 - MARCIA CRISTINA DA COSTA MIGUEL