Banca de DEFESA: VIVIANE ALVES DE OLIVEIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : VIVIANE ALVES DE OLIVEIRA
DATA : 25/01/2018
HORA: 14:30
LOCAL: AUDITÓRIO DO DEPARTAMENTO DE ODONTOLOGIAUFRN
TÍTULO:

IMMUNOHISTOCHEMICAL EXPRESSION OF APE1, XRCC1, P53 AND KI67 PROTEINS IN ORAL TONGUE SQUAMOUS CELL CARCINOMA 

PALAVRAS-CHAVES:

Oral Cancer. Squamous cell carcinoma. DNA repair. Cell proliferation. Immunohistochemistry.

PÁGINAS: 113
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
SUBÁREA: Clínica Odontológica
RESUMO:

Control loss of the process of cell proliferation is considered as one of the precursors of oral cancer. After exposure of the organism to genotoxic agents, DNA repair proteins are produced in order to remove genetic damage. It is believed that the XRCC1 and APE1 proteins which are components of the BER repair pathway are involved in these processes. Thus, the aim of this study was to analyze the immunohistochemical expression of the DNA repair proteins APE1 and XRCC1, and also p53 and ki67, proteins involved in cell cycle, associating them with clinical and histopathological prognostic parameters in oral tongue squamous cell carcinoma (OTSCC), in an attempt to contribute to the better understanding of the participation of these proteins in the development of this neoplasia. The immunohistochemical expression of APE1 and XRCC-1 was evaluated semiquantitatively and that of p53 and ki67 quantitatively in 58 cases of OTSCC. The clinical data were collected in the medical records of each patient and the histopathological grading of Brandwein-Gensler carried out for each case. For the statistical analysis, Chi-square and Fisher's exact tests were performed, and significance was set at p <0.05. Most of the cases presented high immunoexpression for APE1 (n = 36; 62.1%), as well as for XRCC1 (n = 38; 65.5%). For the Ki67 and p53 proteins, there was an equal distribution when the cases were categorized into low and high expression (n = 29, 50%). XRCC1 immunoexpression was significantly higher in cases of early stage I and II lesions (n = 23; 62.2%) compared to advanced stages III and IV (n = 16, 80%, p = 0.05). Immunoexpression of p53 was significantly higher in cases of advanced stage lesion (n = 19; 65.5%) and low in early stages lesions (n = 17, 60.7%, p = 0.047). Neither the proteins studied showed association with each other, nor with the other clinical parameters and histopathological grading. It was observed that there was a significant association of the highest XRCC-1 immunoexpression with better clinical staging and p53 with the worst clinical staging, however, such association was not confirmed when the patients' outcome was analyzed. The results of this experiment indicate that the immunohistochemical expression of XRCC1, APE1, p53 and Ki67 proteins is not associated with prognostic parameters of OTSCC.

MEMBROS DA BANCA:
Externo à Instituição - BETANIA FACHETTI RIBEIRO - UNP
Interno - 1660087 - BRUNO CESAR DE VASCONCELOS GURGEL
Interno - 350485 - HEBEL CAVALCANTI GALVAO
Externo à Instituição - JAMILE MARINHO BEZERRA DE OLIVEIRA MOURA - UERN
Presidente - 350484 - ROSEANA DE ALMEIDA FREITAS