Banca de QUALIFICAÇÃO: MARÍLIA FARIAS DE MELO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARÍLIA FARIAS DE MELO
DATA : 15/02/2019
HORA: 14:00
LOCAL: Sala de reuniões do Centro de Biociências.
TÍTULO:

OUTLINE OF THE PROFILE OF THE ADULT IMMUNE RESPONSE IN PATIENTS INFECTED BY CHIKUNGUNYA FEVER DURING THE ACUTE PHASE IN RIO GRANDE DO NORTE.

PALAVRAS-CHAVES:

Chikungunya; adaptive immune response; T cells; cytokines; transcription factor.

PÁGINAS: 74
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

Chikungunya fever is arbovirus transmitted by arthropods of the genus Aedes, which causes a great economic impact and implications for the world public health. The Chikungunya virus (CHIKV) belongs to the Togaviridae family and to the genus Alphavirus, characterized as simple polarized positive-stranded RNA viruses, enveloped and spherical. In this study, we evaluated the profile of the adaptive immune response presented by patients with CHIKV acute infection by quantifying the expression of transcription factors Tbet, GATA3, PU.1, RORγT, AHR and FOXp3 and the gene expression of cytokines IFNγ, IL-9, IL-9, IL-17, IL-22, IL-10 and TGFβ, with respect to the major subpopulations of T cells, by means of qRT-PCR. Blood samples from 27 patients with previous diagnosis of CHIKV in the acute phase and nine blood samples from healthy individuals, used as control group, were analyzed. CHIKV-infected patients had high and significant levels of Tbet, GATA3, RORγT and FOXp3 transcription factors, as well as expression of cytokines: IFNγ, IL-4, IL-17 and IL-10. Thus, infected patients had a predominantly pro-inflammatory adaptive immune response profile, expressing the Th1, Th2, Th17, and Treg cell subpopulations.
Keywords: Chikungunya; adaptive immune response; T cells; cytokines; transcription factor.

MEMBROS DA BANCA:
Presidente - 1715230 - JOSELIO MARIA GALVAO DE ARAUJO
Interna - 1346635 - JANEUSA TRINDADE DE SOUTO
Interno - 348473 - JOSE VERISSIMO FERNANDES