QUALITY IMPROVEMENT CYCLE TO REDUCE BLOOD CULTURE CONTAMINATION RATES
Blood culture; biological contamination; phlebotomy; total quality management; patient safety.
Introduction: Blood culture is the reference test for the identification of bacteria in blood samples. A contaminated blood culture sample is defined by the presence of microorganisms not present in the patient’s bloodstream, unintentionally introduced during sample collection or processing. False-positive results compromise diagnostic reliability and are associated with increased length of hospital stay and higher healthcare costs. Objective: To test the effectiveness of a quality improvement cycle in reducing blood culture contamination rates in a public hospital in Rio de Janeiro, Brazil. Methods: This was a mixed-methods, quasi-experimental before-and-after study without a control group, conducted during the 2024–2025 biennium and guided by the SQUIRE guidelines. Eleven quality criteria related to potential causes of blood culture contamination were defined. Interventions were participatory, data-driven, and multifaceted, aiming to increase healthcare workers’ adherence to the standard operating procedure for blood culture collection and to the defined quality criteria. Ethical principles in accordance with Resolution No. 466/12 were observed.
Results: The mean post-intervention contamination rate (7.42%) was higher than the pre-intervention rate (6.17%). However, monthly analysis revealed a heterogeneous pattern, with a peak immediately after the intervention followed by a progressive reduction, reaching 2.08% in December, below the recommended threshold, indicating an improving trend by the end of the study period.
Conclusions: The quality improvement cycle demonstrated that enhancing blood culture collection processes requires not only educational interventions but also active engagement from leadership and senior management. The use of an artificial intelligence–based educational video developed as a product of this study aims to support the sustainability of contamination rates as an institutional quality indicator, enabling continuous monitoring and strengthening patient safety initiatives.