Banca de QUALIFICAÇÃO: FIAMMA GLÁUCIA DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : FIAMMA GLÁUCIA DA SILVA
DATE: 15/05/2024
TIME: 09:00
LOCAL: Online via Google Meet
TITLE:

EVALUATION OF THE MULTIFUNCTIONAL THERAPEUTICAL POTENTIAL OF CHITOSAN MICROPARTICLES COTAINING Crotalus durissus cascavella VENOM SNAKE

KEY WORDS:

Bioactive proteins. Snake venom. Antimicrobial. Chitosa nanoparticles.

PAGES: 69
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Antimicrobial resistance (AMR) is a threat to the global population and may be responsible for the emergence and re-emergence of several infectious diseases. Infections caused by multidrug-resistant pathogens present high morbidity and mortality, which has led to an incessant search for new therapeutic alternatives. The South American rattlesnake (Crotalus durissus) contains in its venom several biologically active proteins with important pharmacological action, which can be a valuable tool in the research of new drugs. Polymeric nanocarriers have been investigated as delivery systems for macromolecules. Chitosan, due to its properties, has been extensively investigated in nanocarrier formulations, particularly for proteins and peptides. With this focus, the present study used chitosan nanoparticles (NPQ) as a sustained release system for the Crotalus durissus cascavella venom snake, in order to provide a new pharmacological model. Biocompatibility was evaluated in normal cell lines (Vero E6, and NIH/3T3 and human erythrocytes), demonstrating that in the majority of tested concentrations there was no evidence of hemolytic nor cytotoxic effects.Results demonstrate the nanoparticles ability to inhibit standard gram-positive and gram-negative bacteria and multi-resistant clinical isolates and inhibit different species of the genus Candida. The crude venom and the nanoparticles associated with the venom demonstrated cytotoxicity in the epimastigote and trypomastigote forms of T. cruzi, with late apoptosis and low levels of mitochondrial damage as the main mechanism of action. The leishmanicidal effect was mainly evidenced by NPQ associated with venom, reaching almost 100% inhibition by NPQ with venom incorporated at 1.0%. NPQ without and associated with the venom also demonstrated a larvicidal effect against Aedes aegypti larvae. Thus, this study demonstrated the multifunctional therapeutic potential of chitosan nanoparticles associated or not with venom, as well as crude C.d. cascavella venom, which can be used as a prototype to obtain new drugs with biotechnological application.

COMMITTEE MEMBERS:
Presidente - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externo ao Programa - 2611909 - CARLOS RAMON DO NASCIMENTO BRITO - UFRNExterna ao Programa - 1143827 - JULIANA FELIX DA SILVA - UFRN