Banca de DEFESA: SEBASTIÃO ÂNDERSON DANTAS DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : SEBASTIÃO ÂNDERSON DANTAS DA SILVA
DATE: 29/05/2026
TIME: 14:00
LOCAL: Online (solicitar link à secretaria pelo e-mail renorbio.rn@gmail.com)
TITLE:

Valorization of yellow mombin (Spondias mombin L.) residues for functional bioproduct production and symbiotic microparticle development

 

KEY WORDS:

Residue, Antioxidants, Pretreatment, Ethanol, Functional Foods, Probiotic, Prebiotic, Encapsulation, Taperebá, Xylo-oligosaccharides. 

PAGES: 120
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Agro-industrial waste is a source of numerous bioactive compounds, such as phenolics or functional prebiotics like xylo-oligosaccharides (XOS), which can be used by probiotic bacteria, providing numerous health benefits to consumers. However, this waste is often discarded soon after processing, causing environmental problems and economic losses. This is the case with cajá (Spondias mombin L.), where about 50% of the constituents are considered waste and are eliminated after processing. In this context, the integration of these bioactive compounds into encapsulating matrices creates an ideal symbiotic system, capable of protecting and ensuring the survival of probiotic bacteria against various factors. The present study aimed to valorize cajá waste by extracting bioactive compounds to develop symbiotic microparticles that ensure the viability of the probiotic L. rhamnosus GG. Through hydrothermal and hydroethanolic extraction processes and resin adsorption steps, it was possible to obtain XOS with a yield of 4.0 g/L, predominantly xylotetraose (X4) and various phenolic compounds, such as catechin (19.383 mg/100 g), gallic acid (2.863 mg/100 g), and ferulic acid (1.080 mg/100 g), with confirmed biological safety in toxicity tests. Furthermore, reusing the solid material enabled the production of bioethanol (30.32 g/L) after alkaline pretreatment, thereby increasing the cellulose content and the release of sugars during enzymatic hydrolysis and fermentation. To protect and control the release of these functional compounds and preserve the survival of L. rhamnosus GG, prebiotic (rich in XOS), probiotic, and symbiotic (integrating both functional inputs) microparticles were developed. In general, the microparticles presented diameters between 4.03 and 4.64 μm, with ideal morphological aspects and moderate stability. However, the symbiotic microparticles stood out for their high encapsulation efficiency (83.85%) and cell viability of 12.33 Log CFU/g, surpassing the isolated probiotic formulation. These particles demonstrated high antioxidant capacity against DPPH and ABTS radicals, good antibacterial activity against different pathogens, and antidiabetic properties through the inhibition of α-amylase enzymes (up to 86.25%). Finally, microencapsulation ensured the survival of the strain under critical conditions, maintaining high viability after simulated gastrointestinal digestion (10.61 Log CFU/g) and heat treatments of 63 °C and 73 °C, consolidating the use of cajá waste in the production of high value- added functional products.

 

 

COMMITTEE MEMBERS:
Presidente - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Interna - 1517220 - MARCIA REGINA DA SILVA PEDRINI
Externa à Instituição - NATHALIA KELLY DE ARAUJO - IFRO
Externo à Instituição - PEDRO FERREIRA DE SOUZA FILHO - UFPE
Externo à Instituição - RAPHAEL LUCAS JACINTO ALMEIDA - UFRN