Banca de QUALIFICAÇÃO: SEBASTIÃO ÂNDERSON DANTAS DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : SEBASTIÃO ÂNDERSON DANTAS DA SILVA
DATE: 25/08/2025
TIME: 14:00
LOCAL: https://meet.google.com/swu-wyxq-dmp
TITLE:

Valorization of agroindustrial residue and probiotic application: Biotechnological strategies with yellow mombin (Spondias mombin L.) and Lacticaseibacillus rhamnosus GG

 

KEY WORDS:

Residue, Antioxidants, Pre-treatment, Ethanol, Functional Foods, Probiotic, Encapsulation, Yellow mombin.

 

PAGES: 117
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Agro-industrial waste is a source of numerous bioactive compounds, but it is often discarded soon after processing, causing environmental problems and economic losses. Thus, the yellow mombin is a fruit found in the north and northeast regions of Brazil that is used to manufacture pulp and other products. However, approximately 50% of the constituents are considered waste that is discarded. On the other hand, probiotics are living microorganisms that, when consumed in sufficient quantities, promote considerable effects on the health of their hosts. However, probiotic survival is limited during gastrointestinal digestion and storage, requiring methods such as encapsulation to protect and maintain their cellular viability. Therefore, this study aimed to apply extraction and adsorption techniques to yellow mombin waste to convert it into a high-added-value product, abundant in antioxidant phenolic compounds and bioethanol, and to formulate probiotic microencapsulates with functional action. Bioactive phenolics were recovered by hydroethanolic extraction, which were fractionated and adsorbed on Amberlite XAD7HP resin. The phenolic profile was identified, and the total phenolic compounds and antioxidant activity were quantified. The extracted solids were pretreated with alkali and subjected to enzymatic hydrolysis and semi-simultaneous saccharification and fermentation. On
the other hand, oil-in-water (O/W) emulsification and freeze-drying were used to obtain porcine gelatin, maltodextrin, and chitosan microparticles containing Lacticaseibacillus rhamnosus GG (LRGC), which were characterized and evaluated for their inhibitory capacity against digestive enzymes. The results showed that the extract obtained contained gallic acid (2.863 mg/100 g), ferulic acid (1.080 mg/100 g), and catechin (19.383 mg/100 g). After adsorption, the eluates rich in phenolic compounds presented high antioxidant activity by inhibition of DPPH (158.00 mmol TE/g) and ABTS (1.20 mmol TE/g) radicals. In the solid material remaining from the extraction, pretreated, an increase in cellulose of 18%, crystallinity index of 71%, production of 56.65 g/L of glucose, 11.27 g/L of xylose and 30.32 g/L of ethanol were observed. Furthermore, the microencapsulated probiotic (LRCG) presented a diameter of 4.64 μm, polydispersity index (0.35), surface charge (+19.7 mV), water solubility (54.05%), encapsulation efficiency (78.98%), and cell viability of 9.06 Log Colony Forming Unit per gram (CFU/g). Furthermore, chemical interactions were observed in the microparticles and inhibitory capacity for the enzymes α-amylase (74.38%) and amyloglucosidase (10.81%). Microscopy data indicated the presence of probiotic cells under the surface. Therefore, the biotechnological strategies adopted in this study were ideal for valorizing yellow mombin residue and producing bioactive probiotic microparticles, enabling future industrial applications. 

COMMITTEE MEMBERS:
Presidente - 1517220 - MARCIA REGINA DA SILVA PEDRINI
Externo ao Programa - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA - nullExterno à Instituição - RAPHAEL LUCAS JACINTO ALMEIDA - UFRN