Banca de QUALIFICAÇÃO: ANNE NATÁLIA ALMEIDA DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANNE NATÁLIA ALMEIDA DE OLIVEIRA
DATE: 28/07/2023
TIME: 10:00
LOCAL: Auditório do Química III
TITLE:

Phytochemical study and in vitro enzymatic activity of species of the genus Bredemeyera

KEY WORDS:

Antiophidic; Bothrops leucurus snake; Bredemeyera brevifolia; Bredemeyera floribunda; Flavonoids

PAGES: 100
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

Plant species are popularly used to treat accidents with venomous animals, and their studies are interesting from the phytochemical point of view. The genus Bredemeyera, belonging to the Polygalaceae family, is popularly known as “snake root” due to its use as an antivenom. In addition to its antivenom properties, it is also known for its expectorant, diuretic and hypotensive properties. Thus, the aim of this study was to investigate the phytochemical profile of hydroethanolic extracts from the roots of B. brevifolia and B. floribunda and to eva luate their effects against the main enzymatic activities of the venom of the snake B. leucurus in vitro. HPLC-ESI-Qtrap-MS/MS analysis of hydroethanolic extracts from the roots of B. brevifolia and B. floribunda revealed that flavonoids, xanthones and saponins constitute the most relevant class of secondary metabolites in the extracts. The chromatographic study of this plant resulted in the isolation of two secondary metabolites, the first a flavonoid rutin and the second a xanthone, called 1,7-dihydroxy-3,4,8-trimethoxyxanthone. It also indicated the presence of sugars, in addition to a fraction containing a mixture of two saponins. For the enzymatic inhibition studies, in the proteolytic assay it was observed that the proportions 1:25, 1:50, 1:75 and 1:100 (venom:extract, w/w) both extracts significantly reduced the enzymatic activity. The same way was evidenced in the activity of the phospholipase, all the proportions of the tested extracts proved to be effective in inhibiting the enzymatic effect. In the hyaluronidase method, it was demonstrated that the extract of B. brevifolia significantly prevented the enzymatic activity in the rations 1:2.5, 1:5, 1:10 and 1:25, while the extract of B. floribunda proved to be effective in the rations 1: 10 and 1:25. These dat a revealed the antiophidic potential of the extracts by inhibiting the main enzymatic activities of the B. leucurus snake venom, demonstrating that both extracts can be relevant and promising for the treatment of snake bites.

COMMITTEE MEMBERS:
Externa ao Programa - 1143827 - JULIANA FELIX DA SILVA - nullInterno - 2276354 - LEANDRO DE SANTIS FERREIRA
Presidente - 1569526 - RENATA MENDONÇA ARAUJO