Banca de QUALIFICAÇÃO: TALES GOMES DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : TALES GOMES DA SILVA
DATE: 15/08/2023
TIME: 14:00
LOCAL: videoconferência
TITLE:

DEVELOPMENT OF ANALYTICAL METHODOLOGIES BASED ON ATR FTIR INFRARED SPECTROMETRY AND MASS SPECTROSCOPY WITH MULTIVARIATE ANALYSIS IN THE DIAGNOSIS OF SARCOPENIA

KEY WORDS:

Osteosarcopenia; Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR); Mass Spectroscopy; Multivariate Analysis

PAGES: 85
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SPECIALTY: Métodos Óticos de Análise
SUMMARY:

This thesis demonstrates the development of new analytical methodologies that combine infrared spectroscopy with attenuated total reflection Fourier transform (ATR-FTIR) and instrumental readings with multivariate classification and variable selection, applied to blood plasma samples for detection of the osteosarcopenia. Within the execution of the work, the studies carried out in this thesis explored infrared spectroscopy with attenuated total reflection Fourier transform (ATR-FTIR) and Paper-Spray Ionization Mass Spectrometry (PSI-MS) to obtain spectra from the blood plasma of patients with and without osteosarcopenia, and the processing of these spectral data by multivariate analysis models. In a first study, ATR-FTIR spectroscopy was used to obtain blood plasma spectra from healthy elderly individuals and patients with sarcopenia. Multivariate classification models were constructed, aiming at a diagnostic methodology for screening elderly sarcopenic patients. The PCA-LDA, PCA-QDA, PCA-SVM, SPA-LDA, SPA-QDA and SPA-SVM algorithms were applied as classification tools and their performance compared. In general, the results obtained through the PCA-SVM variable selection algorithm were the most satisfactory, reaching a sensitivity of 89%, thus being able to be related to functional groups belonging to different biomolecules and possible biomarker.

COMMITTEE MEMBERS:
Interna - ***.475.324-** - ANA CAROLINA DE OLIVEIRA NEVES MENEZES - UFRN
Externo à Instituição - CAMILO DE LELIS MEDEIROS DE MORAIS - UECE
Presidente - 1714946 - KASSIO MICHELL GOMES DE LIMA
Externo à Instituição - MARCELO VICTOR DOS SANTOS ALVES - IFRN