Banca de QUALIFICAÇÃO: SARAH POLLYANA DIAS DOS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : SARAH POLLYANA DIAS DOS SANTOS
DATE: 29/05/2023
TIME: 14:00
LOCAL: videoconferência
TITLE:

ASPIDOSPERMA NITIDUM BENTH (APOCYNACEAE): PHYTOCHEMICAL STUDY, EVALUATION OF ACUTE TOXICITY, ANTI-INFLAMMATORY POTENTIAL AND HEALING ACTION

KEY WORDS:

Aspidosperma nitidum; Indole alkaloids; Antiedematogenic; Anti-inflammatory; Security

PAGES: 213
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

Aspidosperma nitidum Benth (Apocynaceae) is a tree found in Brazil mainly in the Amazon region, known as "carapanaúba", being used in popular medicine to treat malaria, leprosy, rheumatism, cancer, diabetes, and inflammatory diseases. However, there are no scientific reports, so far, that prove its popular use as an anti-inflammatory and healing agent. This study aimed to isolate and identify indolic alkaloids, as well as investigate the safety, anti-inflammatory and healing properties of the hydroethanolic extract of the stem bark of Aspidosperma nitidum Benth (ANCCE). The phytochemical study was performed by classical chromatographic methods and by dereplication study using Ultra-Efficient Liquid Chromatography coupled to mass spectrometry (CLUE-EM). Safety was assessed in vitro by 3-methyl-[4-5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay using murine fibroblasts (3T3) and primate renal epithelial cells (Vero E6) and by hemolytic assay, as well as in vivo by acute toxicity model. The anti-edematogenic effect was monitored through carrageenin-induced paw edema model and the anti-inflammatory activity was demonstrated through zymosam-induced air sac model. In addition, the healing potential was evaluated using a skin wound model. In the safety investigation, MTT and hemolytic assays at all concentrations were shown to be non-cytotoxic and acute toxicity model, the use of the extract did not show any signs of toxicity and mortality or significant changes in behavior, biochemical and hematological parameters. In the edematogenic model, ANCCE significantly reduced the percentage of edema, as well as the levels of MPO and pro-inflammatory cytokines. Similarly, for the inflammatory model, the extract proved to be efficient in decreasing leukocyte migrations (mainly polymorphonuclear), as well as reducing the concentrations of total proteins, MPO and cytokines. In addition, ANCCE was effective in reducing the skin wound area during the 7 days of treatment. Our results highlight, for the first time, the anti-inflammatory and healing property of ANCCE, justifying its use in folk medicine. The phytochemical study allowed the isolation of a coumarin, never before isolated from the species, and a glycosylated indole alkaloid, as well as the annotation of 08 (eight) indole alkaloids of the Aspidosperma and b-carboline type, in which this second class of indole alkaloids corroborates with the literature review on b-carboline alkaloids.

COMMITTEE MEMBERS:
Externa à Instituição - MARIA DA CONCEIÇÃO DE MENEZES TÔRRES - UEPB
Interno - 1803692 - FABRICIO GAVA MENEZES
Presidente - 1569526 - RENATA MENDONÇA ARAUJO