The urgency to replace fossil fuels with renewable ones, combined with the growth in biodiesel production and, consequently, glycerol, stimulate research in order to transform biomass-derived products into others with greater added value and, therefore, maintain the production chain. The dehydration reaction of glycerol in gaseous phase using acidic solids is an interesting reaction pathway. To evaluate the viability of this study, catalysts were prepared with different levels of magnesium and zinc spinel dispersed in Beta zeolite and their catalytic properties were analyzed. The different solids were characterized by XRD, FRX, MPE, FTIR, Raman spectroscopy, nitrogen physisorption, SEM-FEG, TPD-NH3 and DRIFTS-pyridine. The Raman and infrared spectra suggested the presence of bands referring to spinels although by XRD the phases were not observed due to their high dispersion and nanometric size. The DRIFTS and NH3-TPD analyzes indicated that the incorporation of spinels modifies the acidic properties regarding the number, type and distribution of the sites. The N2 physisorption tests showed the maintenance of the micro and mesoporous properties of H-BEA, despite the alteration in the specific area and pore volume values. Catalysts with lower spinel content (2%) showed better performances in glycerol conversion and resistance to deactivation when compared to pure zeolite, reaching conversion values of 73.26%. These results are related to the presence of acidic and basic sites in the catalysts. Magnesium-based catalysts, in the glycerol dehydration reactions, favored the formation of acrolein (63.42% for the best material) while zinc-based catalysts produced mostly allelic alcohol (37% for the solid with the best performance) . The reaction pathways for the different products formed, depending on the type of acidic site, were detailed in the proposed mechanisms and correlated with the electrostatic potential maps. Likewise, the reactions for coke formation were described according to the compounds identified by GC-MS after coke extraction.