Banca de DEFESA: ANA KAROLINE SILVA DE AQUINO VITAL

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA KAROLINE SILVA DE AQUINO VITAL
DATE: 25/01/2022
TIME: 14:00
LOCAL: VIDEO CONFERENCIA
TITLE:

“Dereplication of the hydro-ethanolic extract of the roots of Harpalyce brasiliana BENTH (Leguminosae) and evaluation of anti-inflammatory and antioxidant potential”

KEY WORDS:

Anti-inflammatory; Antioxidant; Biocompatibility; Mass Spectrometry; Isoflavonoids; Pterocarpans; Snake’s-root.

PAGES: 90
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

The exacerbated production of reactive oxygen species in the body promotes an imbalance between the amount of oxidizing agents and the removal power of these species in the biological system, generating what is called oxidative stress. Several inflammatory diseases are associated with the overproduction of these species. The hydro-ethanolic extract of Harpalyce brasiliana Benth is widely used by the population in the treatment of snake bites and inflammatory disorders, and this effect is probably associated with the flavonoids present in this species, which are molecules already reported in the literature for their antiophidic properties. Dereplication studies began to be carried out with the aim of avoiding the re-isolation of already known compounds; however, it has become a very useful tool for analyzing the chemical profile of a species of interest. The dereplication of the hydro-ethanolic extract of H. brasiliana, a plant endemic to the Caatinga, popularly known as “snake root”, allowed the identification of 20 compounds by UPLC-MS/MS, mainly prenylated and geranylated flavanones and isoflavones. The extract was also investigated for its biocompatibility, as well as its antioxidant and anti-inflammatory effects. In cell culture, the extract has been shown to be safe in normal cell lines (RAW 247.6 and 3T3) at concentrations of 31-2000 µg/mL. The antioxidant activity of the extract (125-2000 µg/mL) was investigated through assays of reduction of diphenylpicryl-hydrazine, total antioxidant capacity, reducing power, hydroxyl radical elimination and chelation of iron and copper ions. In all in vitro antioxidant tests, the extract showed promising effects. For the dosage of NO (nitric oxide) in cell cultures (RAW 247.6), the extract (500 and 2000 µg/mL) showed a reduction in the expression of NO. In the zymosam-induced air pouch model, mice received zymosam (1 mg/mL) subcutaneously and were subsequently treated (100, 200, and 400 mg/kg) by gavage. In this animal model, the anti-inflammatory effect was observed by reducing the number of leukocytes (especially polymorphonuclear), myeloperoxidase production, total proteins and cytokine levels, as well as demonstrating the antioxidant effect by decreasing malonaldehyde and increasing parameters of glutathione. This approach demonstrates for the first time that the hydro-ethanolic extract from the roots of H. brasiliana is biocompatible, has antioxidant and anti-inflammatory properties in pre-clinical analyses, corroborating its use in folk medicine. Such data draw attention to the species as a promising source of bioactive molecules that can be used in the treatment of inflammatory diseases, which may contribute to the advancement of biopharmaceutical applications for this species.

BANKING MEMBERS:
Interno - 1803692 - FABRICIO GAVA MENEZES
Externo à Instituição - IAN CASTRO GAMBOA - UNESP
Presidente - 1569526 - RENATA MENDONÇA ARAUJO
Externa à Instituição - YANNA CAROLINA FERREIRA TELES - UFPB