Banca de QUALIFICAÇÃO: ANA KAROLINE SILVA DE AQUINO VITAL

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA KAROLINE SILVA DE AQUINO VITAL
DATE: 24/09/2021
TIME: 09:00
LOCAL: videoconferência
TITLE:

Dereplication of the hydroethanolic extract of the roots of Harpalyce brasiliana BENTH (Leguminosae) and evaluation of its anti-inflammatory and antioxidant potential”

KEY WORDS:

Mass Spectrometry; Pterocarpans; Isoflavonoids; Cabenegrin A-II; Anti-inflammatory; Antioxidant; Snake’s-root; Biocompatibility

PAGES: 80
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

The chemistry of natural products has been profoundly modified, especially in relation to the drug discovery process based on molecular targets, which employ large collections of natural and synthetic compounds and their derivatives. Modern tools for the analysis and dereplication of natural products are facilitating the characterization and detection of new bioactive compounds, by excluding already known natural products, and promoting the discovery of more efficient leads, in addition to allowing the suggestion of possible mechanisms of action, based on metabolomics, proteomics and genomics analyses. The dereplication of the hydroethanolic extract of Harpalyce brasiliana, a Caatinga endemic plant popularly known as “snake root”, allowed the identification of 20 compounds by LC-MS/MS, mainly prenylated and geranylated isoflavones and flavanones. The hydroethanolic extract of H. brasiliana is widely used by the population in the treatment of snake bites and inflammatory disorders, and this effect is probably associated with the flavonoids present in this species, which are molecules already reported in the literature for their antiophidic properties. The extract was also investigated in vitro and in vivo for its toxicological potential and was submitted to an evaluation of its antioxidant and anti-inflammatory effects. The extract did not prove to be cytotoxic in erythrocytes and normal cells at the concentrations tested. In all in vitro antioxidant tests, the extract showed promising effects. For NO dosage, the extract showed a reduction in NO expression after extract administration (500 and 2000 µg/mL). The Zimosan air bag model showed a reduction in the number of leukocytes (especially polymorphonuclear), MPO production, total proteins and cytokine levels, as well as demonstrating the antioxidant effect by decreasing MDA and increasing GSH parameters. This approach demonstrates for the first time that the hydroethanolic extract of H. brasiliana roots is biocompatible, has low toxicity and has antioxidant and anti-inflammatory properties in preclinical analyses, corroborating its popular use. Such data draw attention to the species as a promising source of bioactive molecules that can be used in the treatment of inflammatory diseases, which may contribute to the advancement of biopharmaceutical applications for this species.

BANKING MEMBERS:
Externa à Instituição - DÁVILA DE SOUZA ZAMPIERE - UFC
Interno - 1803692 - FABRICIO GAVA MENEZES
Externo à Instituição - JOSÉ DELANO BARRETO MARINHO FILHO
Presidente - 1569526 - RENATA MENDONÇA ARAUJO