Banca de QUALIFICAÇÃO: LIDIANE GONÇALVES DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LIDIANE GONÇALVES DA SILVA
DATE: 11/12/2020
TIME: 14:00
LOCAL: Remotamente pelo google meet
TITLE:

Use of ATR-FTIR spectroscopy combined with multivariate analysis in blood plasma samples as a tool for detecting the HIV virus in pregnant women

KEY WORDS:

HIV; Pregnant women; ATR-FTIR; Multivariate Analysis; GA-LDA.

PAGES: 110
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SPECIALTY: Métodos Óticos de Análise
SUMMARY:

The human immunodeficiency virus (HIV) is the cause of a sexually transmitted infection that can compromise the immune system. The main concern with pregnant women with the virus is the vertical transmission that can occur during pregnancy, during labor and even during the breastfeeding period. The risk of vertical transmission can be reduced through early diagnosis. The colorimetric assays used for the detection of the virus have high costs and are quite laborious, making it necessary to develop fast and low-cost methodologies for the detection of HIV. In this work, infrared spectroscopy with attenuated total reflection Fourier transform (ATR-FTIR) combined with multivariate analysis was used to distinguish HIV-infected patients (n = 40) from healthy controls (n = 80) in a total of 120 blood plasma samples. The PCA-LDA, PCA-QDA, PCA-SVM, GA-LDA, GA-QDA, GA-SVM, SPA-LDA, SPA-QDA and SPA-SVM were used as classification tools. The algorithms had their performances evaluated according to precision, sensitivity, specificity, F-score and G-score. The PCA-SVM, SPA-LDA and GA-LDA algorithms were the ones that obtained the most satisfactory results for the discrimination of the samples with accuracy of 81%, 75% and 88.9% respectively. GA-LDA was the best classification algorithm, presenting the best values of sensitivity (83%), specificity (92%), F-score and G-score (87%). The results obtained demonstrate the potential of ATR-FTIR spectroscopy combined with multivariate analysis as an alternative tool for HIV screening in pregnant women based on blood plasma, being a low-cost, fast and reagent-free method.

BANKING MEMBERS:
Presidente - 1714946 - KASSIO MICHELL GOMES DE LIMA
Interno - 1803692 - FABRICIO GAVA MENEZES
Externo ao Programa - 1913849 - EDGAR PERIN MORAES