Banca de QUALIFICAÇÃO: MAYARA JANE CAMPOS DE MEDEIROS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : MAYARA JANE CAMPOS DE MEDEIROS
DATA : 13/11/2018
HORA: 14:00
LOCAL: Sala de aula do Química 1
TÍTULO:

Spectroscopy, Electrochemical and Antitumor Complexes of Cu(II) with Tridentate Ligands

PALAVRAS-CHAVES:

copper complexes; UV-Vis; IV; NMR; fluorescence; electrochemistry

PÁGINAS: 135
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Inorgânica
ESPECIALIDADE: Química Bio-Inorgânica
RESUMO:

Copper complexes have shown relevant antitumor activity, participating in reactions responsible for DNA breakage and consequent cell death. Thus the present work aims at the synthesis and characterization of 4-({bis[pyridin-2-yl)methyl]amino}methyl)-7-hydroxy-2H-1-benzopyran-2-one (L1) and N,N-Bis(2-hydroxy-naphthylmethyl)methylamine (L2) and the [Cu(phen)(L1)]PF₆ and [Cu(phen)(L2)] complexes, obtained from the precursor [Cu(phen)Cl₂]. The results of IR and NMR indicated the formation of L1 and L2. For the [Cu(phen)(L2)] complex, the IR showed the coordination of L2 to the metal due to the absence of νO-H, whereas for [Cu(phen)(L₁)]PF₆ it was possible to confirm its formation through characteristic vibrational modes. The UV-Vis showed a shift of the d-d transition to the more energetic region compared to the precursor complex. The emission spectra confirmed that the [Cu(phen)(L₁)]PF₆ and [Cu(phen)(L₂)] complexes exhibit fluorescence, even after coordination, whereas the electrochemistry showed for the compound [Cu(phen)(L₂)] two quasi-reversible redox processes Cu^2+/1+ and Cu^1+/0. For the [Cu(phen)(L₁)]PF₆ it was possible to visualize only the process related to the Cu^2+/1+ redox pair in the applied potential range. The cytotoxic potential of the complexes of interest was evaluated with two human tumor lines (A2058 and SiHa) by the MTT assay using the IC₅₀ as the parameter. Similarly to the complexes, the ligands also induced a concentration-dependent cytotoxic effect, however, the complexes demonstrated a highest cytotoxic response. Cell death and cell cycle were also assessed by flow cytometry, where C1phen and C2phen complexes mainly induced late apoptosis. Through the cell cycle, it was possible to conclude that for the SiHa, the C1phen complex inhibited the progression of the cycle in the S phase, whereas for the A2058, both C1phen and also for C2phen, the interruption occurred in the G2-M phase. Therefore, with the biological results in vitro, it is possible to suggest a possible application in the treatment of cancer.

MEMBROS DA BANCA:
Externo ao Programa - 1715109 - DANIEL DE LIMA PONTES
Interno - 1945343 - FRANCISCO ORDELEI NASCIMENTO DA SILVA
Externo ao Programa - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA