Banca de DEFESA: LETÍCIA GRACYELLE ALEXANDRE COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LETÍCIA GRACYELLE ALEXANDRE COSTA
DATA : 07/08/2018
HORA: 10:00
LOCAL: Auditório do NUPPRAR
TÍTULO:

DEVELOPMENT AND VALIDATION OF METHODOLOGY TO DETERMINE ANTITUBERCULOSIS DRUGS IN HOSPITAL EFFLUENT

PALAVRAS-CHAVES:

Hospital effuent; Isoniazid; Rifampicin; Ethambutol; Pirazinamide; HPLC-MS/MS

PÁGINAS: 86
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Analítica
ESPECIALIDADE: Análise de Traços e Química Ambiental
RESUMO:

Drugs are the group of emerging contaminants that have been receiving attention in recent years, with hospital effluents being the predominant source of introduction into the environment. The main concern related to the presence of these compounds in the environment is due to the development of bacterial resistance. The antibiotics stand out because of their intensive use, and it can be emphasized those that fight Tuberculosis considered one of the biggest problems of health worldwide. In view of this conjuncture, it was proposed to develop and validate a liquid chromatography metodology coupled to tandem mass spectrometry with electrospray ionization for the analysis of anti-tuberculosis drugs via direct injection in hospital effluent samples. The developed method complied with all required validation parameters. The method robustness was evaluated through the Youden test, which allowed to determine that the best chromatographic condition was the one using mobile phase flow of 0.150 mL min^-1, column temperature of 25°C, without sample acidification and using Acclain Polar Advanced (C16). The method presented good linearity in the working range of 0.1 to 100 μg L^-1 with R² values higher than 0.9632 for the four drugs. The method was shown to be sensitive, accurate, robust and with recoveries in accordance with INMETRO, above 80%. LDs and LQs were respectively 0.028 and 0.057 μg L^-1 for Pyrazinamide, 0.094 and 0.176 μg L^-1 for Isoniazid, 0.416 and 0.833 μg L^-1 for Rifampicin and 0.263 and 0.519 μg L^-1 for Ethambutol. Regarding Hospital Effluent samples, no Ethambutol was detected in any samples, while Isoniazid was determined in all samples at concentrations between 3.25 and 23.09 μg L^-1. Therefore, it can be concluded that the developed method can be applied for determination of these pharmaceutical compounds with high precision and sensitivity.

MEMBROS DA BANCA:
Presidente - 348475 - DJALMA RIBEIRO DA SILVA
Interno - 347057 - TEREZA NEUMA DE CASTRO DANTAS
Externo ao Programa - 2275848 - ELISAMA VIEIRA DOS SANTOS
Externo à Instituição - SUELY SOUZA LEAL DE CASTRO - UERN