Banca de DEFESA: ARTUR DE SANTANA OLIVEIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ARTUR DE SANTANA OLIVEIRA
DATA : 11/05/2018
HORA: 08:30
LOCAL: Auditório do NUPPRAR
TÍTULO:

DESIGN OF NEW NANOMEDICINES FOR CONTROLLED DRUG DELIVERY OF OLANZAPINE AND CAMPTOTHECIN

PALAVRAS-CHAVES:

Olanzapine, Bionanocomposite, Camptothecin, Covalent Organic Framework, COF-5, Drug Delivery Systems.

PÁGINAS: 90
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Inorgânica
ESPECIALIDADE: Química Bio-Inorgânica
RESUMO:

In recent years, related biomedical studies have been developing with a special emphasis on the controlled drug release from the development of nanostructured materials in exploration of improvements in drug administration. For the development of controlled release systems, we look for factors such as the type of drug, type of administration, resistance of the carrier material in aqueous media, biocompatibility and others. This work was carried out from the studies of the drugs Olanzapine and Camptothecin. Olanzapine (OLZ) is a drug that acts in the treatment of schizophrenia and presents low solubility in aqueous media making it difficult to treat the diseases. The controlled release system for OLZ proposes the incorporation of OLZ into an inorganic support based on montmorillonite (MMT) dispersed in a biopolymer mixture of Alginate (ALG) and Xanthan Gum (GX). After the synthesis of the proposed materials, hybrids and bionanocomposites were characterized by some techniques. The characterizations showed the intercalation of drug in the MMT by ion exchange process, as well as interaction with the biopolymers. The release test was able to show at different pHs that the proposed system shows a more controlled release for the drug studied, than when dissolved only the commercial tablet, indicating that the developed material can act as a controlled release system. For the study of Camptothecin, wide-range spectrum antitumor, the drug was incorporated in a 100% organic matrix through a covalent bonding to COF-5 (Covalent Organic Framework). So, one of the ligands used in the synthesis of COF-5, 1,4-benzenediboronic acid (BDBA), was subjected to nitration and reduction with H2 to provide a functionality with NH2 groups on the pore walls. An esterification with camptothecin and succinic acid was required for subsequent amidation to be obtained successfully. Sequential pore wall modification of covalent organic frameworks (e.g., COF-5) with nucleophyllic groups (e.g., amino) yields highly functionalized materials with outstanding resistance to hydrolytic digestion. Subsequent bonding of camptothecin reports the first case of a stable drug delivery system based on covalently conjugated COFs. The synthesized materials are characterized and tested against the biological fluids. We performed a preliminary study of the biological activity by incubation with HeLa cell line. Cytotoxicity was measured by using the 3-(4,5-dimethylthiazol-2-yl diphenyltetrazolium bromide) (MTT) assay, and calculating the corresponding half maximal inhibitory concentration (IC50) values.

MEMBROS DA BANCA:
Externo à Instituição - ANA CLÉCIA SANTOS DE ALCÂNTARA - UFMA
Externo ao Programa - 1201781 - FERNANDA NERVO RAFFIN
Interno - 1412709 - NEDJA SUELY FERNANDES
Externo à Instituição - PABLO BOTELLA ASUNCIÓN - UPV
Presidente - 1308577 - SIBELE BERENICE CASTELLA PERGHER
Externo à Instituição - VERA R. LEOPOLDO CONSTANTINO - USP