Banca de DEFESA: DANIELE SILVA DOS SANTOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : DANIELE SILVA DOS SANTOS
DATA : 18/08/2017
HORA: 14:00
LOCAL: Auditório do CCET
TÍTULO:

METABOLOMIC ANALYSIS OF Harpalyce brasiliana Benth: Seasonal influence and symbiosis with its endophytic fungi, nigrospora sp, Xylaria sp and colletrotichum sp.

PALAVRAS-CHAVES:

Harpalyce brasiliana, biotic and abiotic stress, xylaria, colletrotichum and nigrospora.

PÁGINAS: 100
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Orgânica
ESPECIALIDADE: Química dos Produtos Naturais
RESUMO:

Harpalyce brasiliana Benth is a typical Northeastern medicinal plant species characterized by its diverse biological activities. The investigation of their secondary metabolites has not been conclusive as to their behavior in the face of seasonal variations and their symbiotic relationship with their endophytic fungi. Thus, this work reports the investigation of the metabolic changes during the entire development of H. brasiliana Benth cultivars through CLUE-EM / QTOF, HPLC-NMR, NMR and CLAE-DAD and chemometric analysis. The use of these techniques allowed the acquisition of spectra of extracts from 90 samples of winter and summer with the advantage of solubility in ethanol, a nontoxic and low cost solvent. The analysis of these spectra through multivariate analysis revealed the high concentration of phloroglucinol (up to 50%), isoflavones (up to 16%) and fatty acid (30%) in the summer period. These have played a key role in the development of the plant acting as antioxidants and osmolytes against a possible oxidative stress caused by temperature variation and solar radiation during plant cultivation. In the winter period it showed a high concentration of glycosylated flavonol and flavone (25 and 47%) and lipid (14%). This tendency of sugars possibly occurred due to the nutrient deficiency caused by the high humidity occurring in the winter, besides serving as a source of carbon and acting as ROS-picking antioxidants. From these leaves, fungi, considered endophytic, nigrospora sp, xylaria sp and colletrotichum sp. Of the extracts, 16 metabolites were identified, some of them found in summer samples, isoflavone and floroglucinol (xylaria sp), coumarin (colletrotichum sp) and oxilipines (nigrospora sp). The identification of these metabolites confirms a symbiotic relationship between these fungi and the plant. They act as osmolytes where the endophytic fungi confer tolerance to the plant in the presence of stresses during the cultivation of their host plant, thus conferring a cross tolerance between plant and fungus with the seasonal variation. Thus, this work contributes to a control of the secondary metabolites with potential biological activity that makes the plant a possible phytotherapic in the cure of diseases that affect the population.

MEMBROS DA BANCA:
Presidente - 1569526 - RENATA MENDONÇA ARAUJO
Interno - 2140775 - LIVIA NUNES CAVALCANTI
Externo ao Programa - 1913849 - EDGAR PERIN MORAES
Externo ao Programa - 1893445 - EUZEBIO GUIMARAES BARBOSA
Externo à Instituição - EDILBERTO ROCHA SILVEIRA - UFC
Externo à Instituição - SAVIO MOITA PINHEIRO - UFPB