Banca de DEFESA: ALINE DE SOUSA MARQUES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ALINE DE SOUSA MARQUES
DATA : 18/08/2017
HORA: 14:00
LOCAL: Auditório do Química 3
TÍTULO:

SPECTROSCOPY AND LIQUID CHROMATOGRAPHY WITH SPECTROMETRY OF MASS ASSOCIATED TO CHEMOMETRY IN THE CLASSIFICATION AND EVALUATION OF LIPIDOMIC PROFILE OF BACTERIAL CLASSES

PALAVRAS-CHAVES:

Bioespectroscopy. SPA-LDA. GA-LDA. NIR. ATR-FTIR. ROI. MCR-ALS. LC-MS

PÁGINAS: 106
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Analítica
ESPECIALIDADE: Métodos Óticos de Análise
RESUMO:

This doctoral thesis is a theoretical-practical contribution for the development of studies that use bioanalytical, particularly biological materials from bacteria, which can be colonies, isolated, DNA, among others, in conjunction with chemistry analysis tools. For this, it was sought to identify bacterial differences when submitted to a source of stress from different analytical techniques. The first approach was based on biospectroscopy, using spectroscopic data obtained in the infrared region. Biospectroscopy in the infrared region is described as a non-invasive, high-throughput, low-cost (when compared with standard analytical techniques) and objective techniques, and has a huge potential in the analysis of bacteria, complementing or even replacing diagnostic methods of diseases conventionally conducted by skilled persons by standard methods of expensive analyzes and requiring specific reagents. The data obtained from biospectroscopy in bacterial samples are complex and have many overlapping bands and it is necessary to apply mathematical tools to overcome these difficulties. For this, some mathematical tools, such as variable selection methods, using Linear Discriminant Analysis with Successive Projection Algorithm (SPA-LDA) and Genetic Algorithm (GA-LDA), are generally used for the purpose of solving these data, facilitating the extraction of information. Infrared spectroscopy, in specific Near Infrared (NIR) and infrared spectroscopy with Fourier transform and Attenuated Total Reflectance (ATR-FTIR), in conjunction with variable selection methods (SPA-LDA and GA-LDA) was used in the discrimination of bacterial samples (Sthaphylococcus aureus, Klebsiella pneumoneae and Pseudomonas aeruginosa). Possible biomarkers such as lipids and proteins were identified at ~ 1550 cm -1 and 1400 cm -1 and DNA vibrations at ~ 1080 cm -1. Sensitivity values of 75% and 95% for SPA-LDA models and 100% and 93% for GA-LDA models were found. Based on these results, it can be concluded that the SPA-LDA and GA-LDA in conjunction with the infrared spectroscopy showed efficient tools improving the time and cost of diagnosis allowing the treatment faster than the standard methods of diagnosis, and consequently, it is possible to avoid the evolution of a possible infection. The second approach was to evaluate possible changes in the lipid profile of bacteria resulting from its exposure to an external stress source (Arsenic (III)), using the cyanobacteria Anabaena sp. and Planktothrix agardhii. The data were obtained from Liquid Chromatography-Mass Spectrometry (LS-MS), which, in order to generate a very extensive data matrix, required the use of a recent selection strategy, defined as ROI (regions of interest), which significantly decreased the Size of the data matrix obtained by LC-MS. Multivariate Curve Resolution - Alternating Least Squares (MCR-ALS) was used as a method to solve variation sources, retrieving the information of its pure components that were mixed. The majority masses found, such as 766.54, 565.40 and 871.56, determine that the studied cyanobacteria, when subjected to As (III), undergo changes related to structures that make up the photosynthetic processes of the same.

MEMBROS DA BANCA:
Interno - 1803692 - FABRICIO GAVA MENEZES
Presidente - 1714946 - KASSIO MICHELL GOMES DE LIMA
Externo ao Programa - 1452833 - MARIA CELESTE NUNES DE MELO
Externo à Instituição - MÁRCIA MIGUEL CASTRO FERREIRA - UNICAMP
Externo à Instituição - MÁRCIO JOSÉ COELHO DE PONTES - UFPB