Banca de QUALIFICAÇÃO: DANIELE SILVA DOS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : DANIELE SILVA DOS SANTOS
DATA : 19/06/2017
HORA: 09:00
LOCAL: Auditório do Química 3
TÍTULO:

Metabolomic approach of Harpalyce brasiliana Benth:  Seasonal Influence and Symbiosis Fungus / Plant

PALAVRAS-CHAVES:

Harpalice Brasiliana, biotic and abiotic stress, Symbiotic fungus plant.

PÁGINAS: 240
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Orgânica
ESPECIALIDADE: Química dos Produtos Naturais
RESUMO:

Harpalyce brasiliana Benth is a species of medicinal plant that has secondary metabolites with diverse biological activities. In this study, its metabolic pathway was analyzed in relation to biotic and abiotic factors applied the metabolomic fingerprint approach with the use of the CLUE-DAD, CLAE-NMR, CLUE-EM/ QTOF and NMR techniques. With the aid of chemometrics the set of data obtained were established to the relationships between environmental factors in the production of secondary metabolites and their symbiotic relationship with endophytic fungi. High temperature and solar radiation were the climatic factors that influenced during the summer cultivation and as an adaptation response the plant increased the production of isoflavones (10.6%), flavonol (24.6%), flavones (53.7%) and phloroglucinois (12.6%). In the winter period, the influence of high air humidity, which led to the accumulation of flavanone (29.5%), glycosylated flavonois (31.5%), pterocarpan (12.6%) and phloroglucinois (11.4%). In the symbiotic interaction, three endophytic fungi were identified, where two of them, nigrospora sp and colletrotichum sp caused the production of new metabolites in the plant. The amines in the winter and summer samples and a phenolic acid only in the summer and the xylaria sp fungus were identified five new bioactive metabolites. The same occurred for the group of unidentified fungi with four isolated fungi the derma sp produced two oxygenated compounds in the plant only in the summer samples, the unidentified fungus sp produced an amine at both times and the fungus apressourim sp produced an amine only in winter.
Resulting in a cross tolerance, where the abiotic factors influence the production of the secondary metabolites and consequently in the symbiotic relationship of the fungus with the plant.

MEMBROS DA BANCA:
Externo ao Programa - 1913849 - EDGAR PERIN MORAES
Interno - 1803692 - FABRICIO GAVA MENEZES
Interno - 1714946 - KASSIO MICHELL GOMES DE LIMA
Presidente - 1569526 - RENATA MENDONÇA ARAUJO