STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF THIOREDOXIN GENES IN Eucalyptus grandis
redoxins, functional characterization, multigenic family, comparative genomics, in silico analysis.
Tree genomes have been sequenced over the past ten years and are a source of basic information for the study of multigenerational families in plants. Comparative genomics, once the complete genome sequences are made available in public banks, is a potent tool to progress with the study of functional gene characterization. In this work, the interest is concentrated in the genes that code thioredoxin and considers the diversity, structure and expression of these genes in the genome of Eucalyptus grandis. For this purpose, bioinformatics tools in public domain platforms were used to identify the coding sequences, to validate the data already obtained with the eucalyptus transcriptome project carried out previously, and to characterize the in silico structure and expression of the genes. The results obtained confirm, through phylogenetic trees containing different thioredoxins from superior plants already sequenced, the presence of multiple sequences in the genome under analysis for all the different types and subgroups of thioredoxins already identified in other genomes. The expression of these genes is distributed in several tissues of the plant, confirming the plasticity and functional complexity of the oxidation-reduction system in plants.