Banca de DEFESA: MARYELLE DE CÁSSIA ALBINO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARYELLE DE CÁSSIA ALBINO
DATE: 29/02/2024
TIME: 08:30
LOCAL: Videoconferência
TITLE:

 

Effects of sucrose and ethanol withdrawal on behaviors related toanxiety and depression: investigation of the role of the KynureninePathway

KEY WORDS:

 

Sucrose;Ethanol;Withdrawal;Indoleamine2,3-dioxygenase;Inulin;Emotional disorders.

PAGES: 96
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

In disorders related to substance use/abuse, the withdrawal phase favors negative reinforcement and continued consumption despite physical and mental harm. Women constitute a risk group for these disorders and few studies investigate changes in neural substrates in females. The enzyme indoleamine 2,3-dioxygenase (IDO), responsible for the metabolism of tryptophan into kynurenines, was seen hyperactivated in males undergoing alcohol withdrawal and associated with emotional disorders. Thus, the present work investigated the effects of sucrose and ethanol withdrawal on behaviors related to anxiety and depression and on IDO activity in brain areas that participate in the modulation of these emotions in Wistar rats. In experiment 1, the rats were subjected to a liquid sucrose diet for 16 days and to behavioral tests on days 3, 5, 22 and 24 after sucrose withdrawal. In experiment 2, females were subjected to an exclusive liquid ethanol diet in increasing concentrations (2, 4 and 6%) for 21 days and to a battery of
behavioral tests on days 22, 23, 24, 25 and 26 after withdrawal of ethanol. The rats that received ethanol were treated with inulin (10 or 30 mg/kg orally, gavage) or mineral water. After the behavioral assessment, the animals were euthanized and had blood and frontal cortex, striatum and hippocampus samples collected. In rats exposed to sucrose there was a reduction in immobility time in the forced swimming test and an increase in plasma triglyceride concentration. No biochemical and behavioral changes were seen in animals on sucrose withdrawal. Rats withdrawing from ethanol exhibited less exploration of open arms in the elevated plus maze, greater grooming in the sucrose spray test, greater burying of glass balls in the marble burying test and greater immobility time in the forced swimming test. There was a trend towards an increase in kynurenine concentration in the cortex. Inulin at doses of 10 and 30 mg/Kg attenuated this increase, and the dose of 30 mg/Kg mitigated the behavioral effects on animals in the elevated plus maze, sucrose spray, marble burying and forced swimming tests. In conclusion, females were not sensitive to sucrose withdrawal. Prolonged consumption of sucrose has demonstrated an antidepressant effect. Ethanol withdrawal promoted anxious and depressive-like behaviors and increased the activity of the kynurenine pathway in females, such changes were reversed by treatment with inulin. The prebiotic demonstrated a potential candidate for the treatment of alcohol use disorder.

COMMITTEE MEMBERS:
Interno - 2412258 - EDILSON DANTAS DA SILVA JUNIOR
Externa ao Programa - 1645202 - ELAINE CRISTINA GAVIOLI - nullExterna à Instituição - ISABELLA MARIA DE OLIVEIRA PONTES FERNANDES - UFERSA
Externa à Instituição - JANAINA MENEZES ZANOVELI - UFPR
Presidente - 1720860 - VANESSA DE PAULA SOARES RACHETTI