Banca de QUALIFICAÇÃO: MARYELLE DE CÁSSIA ALBINO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARYELLE DE CÁSSIA ALBINO
DATE: 03/11/2023
TIME: 08:30
LOCAL: Videoconferência
TITLE:

Effects of ethanol and sucrose withdrawal on behaviors related to anxiety and depression: investigating the role of the kynurenine pathway

KEY WORDS:

Sucrose; alcohol; withdrawal; indoleamine 2,3-dioxygenase; inulin; emotional disorders.

PAGES: 115
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Substance use/abuse disorders and binge eating disorder share neural substrates, neurotransmission systems and behavioral patterns. Previous studies have demonstrated that short- and long-term ethanol withdrawal promoted behavioral changes related to anxiety and depression, associated with changes in the brain activity of the enzyme indoleamine 2,3-dioxygenase (IDO), responsible for the metabolism of tryptophan into kynurenines. The objective of the present study was to investigate the effects of sucrose withdrawal, as well as ethanol, on behaviors associated with anxiety and depression, as well as on IDO activity in the cortex, prefrontal, hippocampus and striatum of females. In experiment 1, Wistar rats were subjected to sucrose (5%) for 16 days, followed by short-term (48 hours) or long-term (21 days) withdrawal prior to open field behavioral tests (3rd or 22nd day of withdrawal) and forced swimming (5th or 24th day of withdrawal). In experiment 2, Wistar rats were subjected to ethanol in increasing concentrations (2, 4 and 6%) for 21 days. On the 14th day after treatment with ethanol, some of the animals received the prebiotic inulin (10% or 30% orally). All ethanol-treated groups underwent long-term withdrawal (21 to 25 days) and behavioral tests in the elevated plus maze (21st day of withdrawal), sucrose spray (22nd day of withdrawal), marble burying (23rd day of withdrawal), pre-test and forced swimming test (respectively 24th and 25th days of withdrawal). Subsequently, the animals were euthanized to obtain blood and brain samples, including parts of the prefrontal cortex, striatum and hippocampus. In experiment 1, rats that received sucrose showed shorter immobility time in the forced swimming test, suggesting an antidepressant-like effect of sugar. The group that received sucrose had a higher concentration of triglycerides in the serum when compared to the control group. There were no changes in glycemic indexes, renal and hepatic function, as well as no changes in IDO activity and oxidative stress markers in rats that drank sucrose, whether or not they underwent withdrawal. In experiment 2, preliminary data analysis suggested that ethanol withdrawal promoted behavioral changes related to anxiety and depression, which were attenuated by inulin treatment.

COMMITTEE MEMBERS:
Externa à Instituição - CLARISSA DE ALMEIDA MOURA
Presidente - 2412258 - EDILSON DANTAS DA SILVA JUNIOR
Externo à Instituição - RAMÓN HYPOLITO LIMA - IIN-ELS