"Production, recovery and purification of chitosanases using Aspergillus fumigatus and Bacillus cereus."
Quitooligosaccharides, chitosanases, purification of biomolecules
Chitosan-based biomaterials constitute an emerging class with application in various biomedical fields. However, it is high in molecular weight and insoluble in water and most organic solvents. Consequently, it is interesting to obtain products resulting from the degradation of chitosan: chito-oligosacc harides (QOS). Chitosan oligosaccharides have several biological properties, such as antitumor, antimicrobial and prebiotic activity. They are obtained from chemical or enzymatic hydrolysis, in the latter case, using glycosyl hydrolases such as chitosanases, chitinases, and other non-specific enzymes. Chitosanases can be produced by a wide variety of microorganisms, such as bacteria and fungi. The isolation of microorganisms that may have an industrial interest represents an important source of raw material for the most varied industrial processes. Thus, considering the high industrial applicability of quitooligosa ccharides (QOS), the present work aims at obtaining and purifying chitosanases produced by microorganisms Bacillus cereus and Aspergillus fumigatus from submerged fermentation.