Banca de QUALIFICAÇÃO: LUCAS DE FREITAS LACERDA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCAS DE FREITAS LACERDA
DATE: 30/11/2023
TIME: 09:00
LOCAL: Google Meet, https://meet.google.com/rwz-prmh-ufj
TITLE:

BIOINFORMATICS APPLICATIONS FOR CONSERVATION OF SAPAJUS GENUS: INTEGRATED MACHINE LEARNING, PHYLOGENETICS AND POPULATION GENETICS ANALYSIS

KEY WORDS:

Hybridization; Genetic Markers; Phylogeny; Landscape Genetics.

PAGES: 74
BIG AREA: Ciências Biológicas
AREA: Genética
SUMMARY:

The anthropogenic pressures experienced by the remnants of the Atlantic Forest on the northeastern coast of Brazil reflect impacts on the conservation status of the animals that make up its fauna, among which are the Neotropical primates. Aiming at the conservation of threatened primates in the Northeast, the CPB/ICMBio coordinates the National Action Plan for the Conservation of the Northeastern Primates (PAN PRINE). One of the target species is the blonde capuchin monkey (Sapajus flavius), categorized as Endangered. Aiming to contribute to the implementation of PAN PRINE actions, this study aimed to build a panel of genetic markers to differentiate two closely related species of the Sapajus genus, understand the diversification and evolutionary relationships among the S. flavius populations through migration analyses with tree-based tests, describe genetic diversity metrics for all putative species populations, assess inbreeding metrics and, finally, test the correlation of the species' movement resistance in land use and cover elements with genetic distance based on population differentiation metrics in a landscape genetics framework. We were able to identify a total of 87 genetic markers from an initial amount of 2603 through three distinct machine learning methodologies, of these methodologies only 13 markers had any intersection between them, proving to be highly informative variants for group differentiation. Phylogenetic analyses demonstrated interspecific introgression of S. libidinosus in the populations north of the S. flavius distribution, in addition to pointing to a scenario of ancestral gene exchange between some populations within the S. flavius species. Genetic diversity metrics showed low levels of diversity and high inbreeding probability among some S. flavius populations, denoting a need for species management, one population was identified as a candidate to be a source of diversity and a possible management target due to the best values found when assessing diversity and inbreeding rates among individuals. The geographical distance between populations appears to have been the most correlated factor with their degrees of differentiation when compared to land use and cover elements. These results are important and serve as initial guidelines to support action plans and management decisions that take into account the genetic profile of the populations and species studied for more assertive conservation measures.

COMMITTEE MEMBERS:
Externa à Instituição - CAMILA JUNQUEIRA MAZZONI
Externa à Instituição - CAROLINA DA SILVA CARVALHO
Externa à Instituição - AMELY BRANQUINHO MARTINS
Presidente - 3063244 - TETSU SAKAMOTO