Banca de QUALIFICAÇÃO: EPITÁCIO DANTAS DE FARIAS FILHO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : EPITÁCIO DANTAS DE FARIAS FILHO
DATE: 28/02/2023
TIME: 14:00
LOCAL: Híbrido, Auditório do BioMe e Google Meet
TITLE:

Transcriptional Signature from Clear Cell Renal Cell Carcinoma based on the competitive endogenous RNA

KEY WORDS:

Transcriptional signature; ceRNA network; feature selection; metastasis; renal carcinoma.

PAGES: 86
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Renal carcinoma, as it is a pathology of silent and multifactorial development, is characterized by a high rate of patients with metastases. After several studies have elucidated the activity of coding genes in the metastatic development of renal carcinoma, new studies seek to evaluate the association of non-coding genes, such as competitive endogenous RNA (ceRNA), with the metastatic process. Thus, the aim of this study is to build a transcriptional signature for clear cell renal cell carcinoma (ccRCC) associated with metastatic development from a ceRNA network and to analyze the probable biological functions performed by the participants of the signature. Using ccRCC data from The Cancer Genome Atlas (TCGA), we constructed nine transcriptional signatures from eight feature selection techniques and analyzed the sensitivity and specificity of prediction of regression models in the benchmarking process. Consequently, signature genes were obtained and analyzes of somatic and copy number changes, risk analysis for survival and metastatic progression, and functional enrichment analysis were performed. In this study we present a transcriptional signature of 10 genes, composed of 2 long non-coding RNAs, SNHG15 and AF117829.1, 2 miRNAs, hsa-miR-130a-3p and hsa-mir-381-3p, and 6 mRNAs, BTBD11, INSR, HECW2, RFLNB, PTTG1, HMMR. Genomic analysis identified that the signature participants are located on chromosomes with highly mutated regions (G-index > 2). The hsa-miR-130a-3p genes, AF117829.1 and HECW2, had a significant relationship between expression and patient survival, and the last two have a significant relationship with metastatic development. In addition, functional enrichment was seen in important pathways for tumor development, such as: PI3K/AKT, TNF, FoxO, RNA polymerase 2 transcription regulation, cell control, and others. Finally, by analyzing the connections of the signature genes within the ceRNA network in conjunction with studies in the literature, it was possible to obtain an overview of the activities performed by them within the ccRCC. Therefore, this transcriptional signature can identify non-coding genes as potential biomarkers to be used for a better understanding of renal carcinoma, as well as in the development of future treatments in the clinical area.

COMMITTEE MEMBERS:
Externo à Instituição - ALEXANDRE ROSSI PASCHOAL
Presidente - 1365498 - BEATRIZ STRANSKY FERREIRA
Externo ao Programa - 3884005 - PATRICK CESAR ALVES TERREMATTE - nullInterno - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN