Banca de DEFESA: BRUNO MATTOS SILVA WANDERLEY

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : BRUNO MATTOS SILVA WANDERLEY
DATA : 25/11/2019
HORA: 09:00
LOCAL: Instituto Metrópole Digital (IMD) Sala B 206
TÍTULO:

flowDiv: A New Pipeline for Analyzing Flow Cytometric Diversity

PALAVRAS-CHAVES:

Flow cytometry, cytometric diversity, flowDiv.

PÁGINAS: 54
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Flow cytometry (FCM) is an analytical technique based on the spectroscopic characterization of particulates. This technique allows the quantitative and qualitative description of a wide range of cellular systems within seconds and at relatively low costs. Such features make it a very ubiquitous tool in both industrial and academic analytical protocols. The environmental sciences have been dealing with quite obvious obstacles with regrads to the structuring of FCM protocols: the highly heterogeneous nature of environmental samples makes it difficult to adjust protocols that balance standard mathematical reasoning and the intrinsic biological meanings of the system under study. Several approaches have been devised to correct these incongruities, including those that explore the idea of cytometric diversity - the study of FCM data based on numerical ecology methods - has been quite auspicious. However, despite the availability of solutions, many technical challenges still need to be overcome. In this work, we develop and apply a new computational tool, flowDiv, specially designed for the analysis of cytometric diversity of environmental data. Here, in addition to detailing the logic behind the method and comparing it to similar computational strategies, we apply it to real problems, revealing how some important ecological factors, such as nutritional status, affect the cytometric diversity of microbial groups in natural lakes at Patagonian Argentina and northeast Brazil.

MEMBROS DA BANCA:
Presidente - 347628 - ADRIAO DUARTE DORIA NETO
Interno - 1669545 - DANIEL SABINO AMORIM DE ARAUJO
Interno - 2170415 - JORGE ESTEFANO SANTANA DE SOUZA
Externo à Instituição - ANDRE MEGALI AMADO - UFJF
Externo à Instituição - FERNANDO UNREIN
Externo à Instituição - ROSEMBERG FERNANDES DE MENEZES - UFPB